Practical Recommendations for Improving Efficiency and Accuracy of the CRISPR/Cas9 Genome Editing System
- 作者: Karagyaur M.1, Rubtsov Y.1, Vasiliev P.2, Tkachuk V.1
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隶属关系:
- Lomonosov Moscow State University
- Research Center of Medical Genetics
- 期: 卷 83, 编号 6 (2018)
- 页面: 629-642
- 栏目: Review
- URL: https://journals.rcsi.science/0006-2979/article/view/151665
- DOI: https://doi.org/10.1134/S0006297918060020
- ID: 151665
如何引用文章
详细
CRISPR/Cas9 genome-editing system is a powerful, fairly accurate, and efficient tool for modifying genomic DNA. Despite obvious advantages, it is not devoid of certain drawbacks, such as propensity for introduction of additional nonspecific DNA breaks, insufficient activity against aneuploid genomes, and relative difficulty in delivering its components to cells. In this review, we focus on the difficulties that can limit the use of CRISPR/Cas9 and suggest a number of practical recommendations and information sources that will make it easier for the beginners to work with this outstanding technological achievement of the XXI century.
作者简介
M. Karagyaur
Lomonosov Moscow State University; Lomonosov Moscow State University
编辑信件的主要联系方式.
Email: m.karagyaur@mail.ru
俄罗斯联邦, Moscow, 119192; Moscow, 119192
Y. Rubtsov
Lomonosov Moscow State University
Email: m.karagyaur@mail.ru
俄罗斯联邦, Moscow, 119192
P. Vasiliev
Research Center of Medical Genetics
Email: m.karagyaur@mail.ru
俄罗斯联邦, Moscow, 115478
V. Tkachuk
Lomonosov Moscow State University; Lomonosov Moscow State University
Email: m.karagyaur@mail.ru
俄罗斯联邦, Moscow, 119192; Moscow, 119192
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