Practical Recommendations for Improving Efficiency and Accuracy of the CRISPR/Cas9 Genome Editing System
- Authors: Karagyaur M.N.1, Rubtsov Y.P.1, Vasiliev P.A.2, Tkachuk V.A.1
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Affiliations:
- Lomonosov Moscow State University
- Research Center of Medical Genetics
- Issue: Vol 83, No 6 (2018)
- Pages: 629-642
- Section: Review
- URL: https://journals.rcsi.science/0006-2979/article/view/151665
- DOI: https://doi.org/10.1134/S0006297918060020
- ID: 151665
Cite item
Abstract
CRISPR/Cas9 genome-editing system is a powerful, fairly accurate, and efficient tool for modifying genomic DNA. Despite obvious advantages, it is not devoid of certain drawbacks, such as propensity for introduction of additional nonspecific DNA breaks, insufficient activity against aneuploid genomes, and relative difficulty in delivering its components to cells. In this review, we focus on the difficulties that can limit the use of CRISPR/Cas9 and suggest a number of practical recommendations and information sources that will make it easier for the beginners to work with this outstanding technological achievement of the XXI century.
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About the authors
M. N. Karagyaur
Lomonosov Moscow State University; Lomonosov Moscow State University
Author for correspondence.
Email: m.karagyaur@mail.ru
Russian Federation, Moscow, 119192; Moscow, 119192
Y. P. Rubtsov
Lomonosov Moscow State University
Email: m.karagyaur@mail.ru
Russian Federation, Moscow, 119192
P. A. Vasiliev
Research Center of Medical Genetics
Email: m.karagyaur@mail.ru
Russian Federation, Moscow, 115478
V. A. Tkachuk
Lomonosov Moscow State University; Lomonosov Moscow State University
Email: m.karagyaur@mail.ru
Russian Federation, Moscow, 119192; Moscow, 119192
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