Analysis of free amino acids in mammalian brain extracts
- 作者: Ksenofontov A.1, Boyko A.2, Mkrtchyan G.2, Tashlitsky V.3, Timofeeva A.1, Graf A.4,5, Bunik V.1,3, Baratova L.1
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隶属关系:
- Belozersky Institute of Physico-Chemical Biology
- Lomonosov Moscow State University, Faculty of Bioengineering and Bioinformatics
- Lomonosov Moscow State University, Faculty of Chemistry
- Lomonosov Moscow State University, Faculty of Biology
- Faculty of Nano-, Bio-, Informational and Cognitive Technologies at Moscow Institute of Physics and Technology
- 期: 卷 82, 编号 10 (2017)
- 页面: 1183-1192
- 栏目: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151487
- DOI: https://doi.org/10.1134/S000629791710011X
- ID: 151487
如何引用文章
详细
An optimized method for analysis of free amino acids using a modified lithium-citrate buffer system with a Hitachi L-8800 amino acid analyzer is described. It demonstrates clear advantages over the sodium-citrate buffer system commonly used for the analysis of protein hydrolysates. A sample pretreatment technique for amino acid analysis of brain extracts is also discussed. The focus has been placed on the possibility of quantitative determination of the reduced form of glutathione (GSH) with simultaneous analysis of all other amino acids in brain extracts. The method was validated and calibration coefficient (KGSH) was determined. Examples of chromatographic separation of free amino acids in extracts derived from different parts of the brain are presented.
作者简介
A. Ksenofontov
Belozersky Institute of Physico-Chemical Biology
编辑信件的主要联系方式.
Email: ksenofon@belozersky.msu.ru
俄罗斯联邦, Moscow, 119991
A. Boyko
Lomonosov Moscow State University, Faculty of Bioengineering and Bioinformatics
Email: baratova@belozersky.msu.ru
俄罗斯联邦, Moscow, 119991
G. Mkrtchyan
Lomonosov Moscow State University, Faculty of Bioengineering and Bioinformatics
Email: baratova@belozersky.msu.ru
俄罗斯联邦, Moscow, 119991
V. Tashlitsky
Lomonosov Moscow State University, Faculty of Chemistry
Email: baratova@belozersky.msu.ru
俄罗斯联邦, Moscow, 119991
A. Timofeeva
Belozersky Institute of Physico-Chemical Biology
Email: baratova@belozersky.msu.ru
俄罗斯联邦, Moscow, 119991
A. Graf
Lomonosov Moscow State University, Faculty of Biology; Faculty of Nano-, Bio-, Informational and Cognitive Technologies at Moscow Institute of Physics and Technology
Email: baratova@belozersky.msu.ru
俄罗斯联邦, Moscow, 119991; Moscow, 123098
V. Bunik
Belozersky Institute of Physico-Chemical Biology; Lomonosov Moscow State University, Faculty of Chemistry
Email: baratova@belozersky.msu.ru
俄罗斯联邦, Moscow, 119991; Moscow, 119991
L. Baratova
Belozersky Institute of Physico-Chemical Biology
编辑信件的主要联系方式.
Email: baratova@belozersky.msu.ru
俄罗斯联邦, Moscow, 119991