Analysis of free amino acids in mammalian brain extracts
- Authors: Ksenofontov A.L.1, Boyko A.I.2, Mkrtchyan G.V.2, Tashlitsky V.N.3, Timofeeva A.V.1, Graf A.V.4,5, Bunik V.I.1,3, Baratova L.A.1
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Affiliations:
- Belozersky Institute of Physico-Chemical Biology
- Lomonosov Moscow State University, Faculty of Bioengineering and Bioinformatics
- Lomonosov Moscow State University, Faculty of Chemistry
- Lomonosov Moscow State University, Faculty of Biology
- Faculty of Nano-, Bio-, Informational and Cognitive Technologies at Moscow Institute of Physics and Technology
- Issue: Vol 82, No 10 (2017)
- Pages: 1183-1192
- Section: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151487
- DOI: https://doi.org/10.1134/S000629791710011X
- ID: 151487
Cite item
Abstract
An optimized method for analysis of free amino acids using a modified lithium-citrate buffer system with a Hitachi L-8800 amino acid analyzer is described. It demonstrates clear advantages over the sodium-citrate buffer system commonly used for the analysis of protein hydrolysates. A sample pretreatment technique for amino acid analysis of brain extracts is also discussed. The focus has been placed on the possibility of quantitative determination of the reduced form of glutathione (GSH) with simultaneous analysis of all other amino acids in brain extracts. The method was validated and calibration coefficient (KGSH) was determined. Examples of chromatographic separation of free amino acids in extracts derived from different parts of the brain are presented.
About the authors
A. L. Ksenofontov
Belozersky Institute of Physico-Chemical Biology
Author for correspondence.
Email: ksenofon@belozersky.msu.ru
Russian Federation, Moscow, 119991
A. I. Boyko
Lomonosov Moscow State University, Faculty of Bioengineering and Bioinformatics
Email: baratova@belozersky.msu.ru
Russian Federation, Moscow, 119991
G. V. Mkrtchyan
Lomonosov Moscow State University, Faculty of Bioengineering and Bioinformatics
Email: baratova@belozersky.msu.ru
Russian Federation, Moscow, 119991
V. N. Tashlitsky
Lomonosov Moscow State University, Faculty of Chemistry
Email: baratova@belozersky.msu.ru
Russian Federation, Moscow, 119991
A. V. Timofeeva
Belozersky Institute of Physico-Chemical Biology
Email: baratova@belozersky.msu.ru
Russian Federation, Moscow, 119991
A. V. Graf
Lomonosov Moscow State University, Faculty of Biology; Faculty of Nano-, Bio-, Informational and Cognitive Technologies at Moscow Institute of Physics and Technology
Email: baratova@belozersky.msu.ru
Russian Federation, Moscow, 119991; Moscow, 123098
V. I. Bunik
Belozersky Institute of Physico-Chemical Biology; Lomonosov Moscow State University, Faculty of Chemistry
Email: baratova@belozersky.msu.ru
Russian Federation, Moscow, 119991; Moscow, 119991
L. A. Baratova
Belozersky Institute of Physico-Chemical Biology
Author for correspondence.
Email: baratova@belozersky.msu.ru
Russian Federation, Moscow, 119991