Glutamic acid as enhancer of protein synthesis kinetics in hepatocytes from old rats
- 作者: Brodsky V.1, Malchenko L.1, Butorina N.1, Lazarev (Konchenko) D.2, Zvezdina N.1, Dubovaya T.2
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隶属关系:
- Koltsov Institute of Developmental Biology
- Pirogov Russian State Medical University
- 期: 卷 82, 编号 8 (2017)
- 页面: 957-961
- 栏目: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151459
- DOI: https://doi.org/10.1134/S0006297917080119
- ID: 151459
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详细
Dense cultures of hepatocytes from old rats (~2 years old, body weight 530-610 g) are different from similar cultures of hepatocytes from young rats by the low amplitude of protein synthesis rhythm. Addition of glutamic acid (0.2, 0.4, or 0.6 mg/ml) into the culture medium with hepatocytes of old rats resulted in increase in the oscillation amplitudes of the protein synthesis rhythm to the level of young rats. A similar action of glutamic acid on the protein synthesis kinetics was observed in vivo after feeding old rats with glutamic acid. Inhibition of metabotropic receptors of glutamic acid with α-methyl-4-carboxyphenylglycine (0.01 mg/ml) abolished the effect of glutamic acid. The amplitude of oscillation of the protein synthesis rhythm in a cell population characterizes synchronization of individual oscillations caused by direct cell–cell communications. Hence, glutamic acid, acting as a receptor-dependent transmitter, enhanced direct cell–cell communications of hepatocytes that were decreased with aging. As differentiated from other known membrane signaling factors (gangliosides, norepinephrine, serotonin, dopamine), glutamic acid can penetrate into the brain and thus influence the communications and protein synthesis kinetics that are disturbed with aging not only in hepatocytes, but also in neurons.
作者简介
V. Brodsky
Koltsov Institute of Developmental Biology
编辑信件的主要联系方式.
Email: brodsky.idb@bk.ru
俄罗斯联邦, Moscow, 117808
L. Malchenko
Koltsov Institute of Developmental Biology
Email: brodsky.idb@bk.ru
俄罗斯联邦, Moscow, 117808
N. Butorina
Koltsov Institute of Developmental Biology
Email: brodsky.idb@bk.ru
俄罗斯联邦, Moscow, 117808
D. Lazarev (Konchenko)
Pirogov Russian State Medical University
Email: brodsky.idb@bk.ru
俄罗斯联邦, Moscow, 117513
N. Zvezdina
Koltsov Institute of Developmental Biology
Email: brodsky.idb@bk.ru
俄罗斯联邦, Moscow, 117808
T. Dubovaya
Pirogov Russian State Medical University
Email: brodsky.idb@bk.ru
俄罗斯联邦, Moscow, 117513
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