“Suppressor factor” of neutrophils: A short story of a long-term misconception
- 作者: Linge I.1, Kondratieva E.1, Kondratieva T.1, Makarov V.2, Polshakov V.3, Savelyev O.3, Apt A.1
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隶属关系:
- Central Research Institute for Tuberculosis
- Institute of Molecular Medicine
- Faculty of Fundamental Medicine, Center for Magnetic Tomography and Spectroscopy
- 期: 卷 81, 编号 11 (2016)
- 页面: 1284-1292
- 栏目: Molecular and Cellular Mechanisms of Inflammation (Special Issue) Guest Editors S. A. Nedospasov and D. V. Kuprash
- URL: https://journals.rcsi.science/0006-2979/article/view/151082
- DOI: https://doi.org/10.1134/S0006297916110067
- ID: 151082
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详细
A large body of evidence obtained during the last decade has demonstrated that neutrophils suppress T cell proliferation in different models of inflammation and cell interaction. The commonly used method for assessing cell proliferation and proliferation inhibition is measuring [3H]thymidine incorporation into cells. Earlier, we observed inhibition of [3H]thymidine uptake in experiments on neutrophil-mediated regulation of T cell response in tuberculosis immunity. Here, we used different types of proliferating cells to analyze the nature of the soluble “neutrophil factor” by a variety of methods (dialysis, HPLC, mass spectrometry, and NMR) and unambiguously demonstrated that neutrophils do not synthesize a specific factor inhibiting cell proliferation, but secrete high concentrations of extracellular thymidine that competitively inhibit [3H]thymidine incorporation. Although the physiological significance of thymidine secretion by neutrophils remains unknown, this phenomenon should be carefully considered when designing test systems for studying cell–cell interactions.
作者简介
I. Linge
Central Research Institute for Tuberculosis
编辑信件的主要联系方式.
Email: iralinge@gmail.com
俄罗斯联邦, Moscow, 107564
E. Kondratieva
Central Research Institute for Tuberculosis
Email: asapt@aha.ru
俄罗斯联邦, Moscow, 107564
T. Kondratieva
Central Research Institute for Tuberculosis
Email: asapt@aha.ru
俄罗斯联邦, Moscow, 107564
V. Makarov
Institute of Molecular Medicine
Email: asapt@aha.ru
俄罗斯联邦, Moscow, 119991
V. Polshakov
Faculty of Fundamental Medicine, Center for Magnetic Tomography and Spectroscopy
Email: asapt@aha.ru
俄罗斯联邦, Moscow, 119991
O. Savelyev
Faculty of Fundamental Medicine, Center for Magnetic Tomography and Spectroscopy
Email: asapt@aha.ru
俄罗斯联邦, Moscow, 119991
A. Apt
Central Research Institute for Tuberculosis
编辑信件的主要联系方式.
Email: asapt@aha.ru
俄罗斯联邦, Moscow, 107564