“Suppressor factor” of neutrophils: A short story of a long-term misconception
- Authors: Linge I.A.1, Kondratieva E.V.1, Kondratieva T.K.1, Makarov V.A.2, Polshakov V.I.3, Savelyev O.Y.3, Apt A.S.1
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Affiliations:
- Central Research Institute for Tuberculosis
- Institute of Molecular Medicine
- Faculty of Fundamental Medicine, Center for Magnetic Tomography and Spectroscopy
- Issue: Vol 81, No 11 (2016)
- Pages: 1284-1292
- Section: Molecular and Cellular Mechanisms of Inflammation (Special Issue) Guest Editors S. A. Nedospasov and D. V. Kuprash
- URL: https://journals.rcsi.science/0006-2979/article/view/151082
- DOI: https://doi.org/10.1134/S0006297916110067
- ID: 151082
Cite item
Abstract
A large body of evidence obtained during the last decade has demonstrated that neutrophils suppress T cell proliferation in different models of inflammation and cell interaction. The commonly used method for assessing cell proliferation and proliferation inhibition is measuring [3H]thymidine incorporation into cells. Earlier, we observed inhibition of [3H]thymidine uptake in experiments on neutrophil-mediated regulation of T cell response in tuberculosis immunity. Here, we used different types of proliferating cells to analyze the nature of the soluble “neutrophil factor” by a variety of methods (dialysis, HPLC, mass spectrometry, and NMR) and unambiguously demonstrated that neutrophils do not synthesize a specific factor inhibiting cell proliferation, but secrete high concentrations of extracellular thymidine that competitively inhibit [3H]thymidine incorporation. Although the physiological significance of thymidine secretion by neutrophils remains unknown, this phenomenon should be carefully considered when designing test systems for studying cell–cell interactions.
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About the authors
I. A. Linge
Central Research Institute for Tuberculosis
Author for correspondence.
Email: iralinge@gmail.com
Russian Federation, Moscow, 107564
E. V. Kondratieva
Central Research Institute for Tuberculosis
Email: asapt@aha.ru
Russian Federation, Moscow, 107564
T. K. Kondratieva
Central Research Institute for Tuberculosis
Email: asapt@aha.ru
Russian Federation, Moscow, 107564
V. A. Makarov
Institute of Molecular Medicine
Email: asapt@aha.ru
Russian Federation, Moscow, 119991
V. I. Polshakov
Faculty of Fundamental Medicine, Center for Magnetic Tomography and Spectroscopy
Email: asapt@aha.ru
Russian Federation, Moscow, 119991
O. Yu. Savelyev
Faculty of Fundamental Medicine, Center for Magnetic Tomography and Spectroscopy
Email: asapt@aha.ru
Russian Federation, Moscow, 119991
A. S. Apt
Central Research Institute for Tuberculosis
Author for correspondence.
Email: asapt@aha.ru
Russian Federation, Moscow, 107564