New fluorescent macrolide derivatives for studying interactions of antibiotics and their analogs with the ribosomal exit tunnel
- 作者: Tereshchenkov A.1, Shishkina A.2, Karpenko V.1, Chertkov V.1, Konevega A.3,4, Kasatsky P.3,4, Bogdanov A.1,2, Sumbatyan N.1
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隶属关系:
- Faculty of Chemistry
- Belozersky Institute of Physico-Chemical Biology
- Molecular and Radiation Biophysics Division, Konstantinov Petersburg Nuclear Physics Institute
- Peter the Great Saint-Petersburg Polytechnic University
- 期: 卷 81, 编号 10 (2016)
- 页面: 1163-1172
- 栏目: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151045
- DOI: https://doi.org/10.1134/S0006297916100138
- ID: 151045
如何引用文章
详细
Novel fluorescent derivatives of macrolide antibiotics related to tylosin bearing rhodamine, fluorescein, Alexa Fluor 488, BODIPY FL, and nitrobenzoxadiazole (NBD) residues were synthesized. The formation of complexes of these compounds with 70S E. coli ribosomes was studied by measuring the fluorescence polarization depending on the ribosome amount at constant concentration of the fluorescent substance. With the synthesized fluorescent tylosin derivatives, the dissociation constants for ribosome complexes with several known antibiotics and macrolide analogs previously obtained were determined. It was found that the fluorescent tylosin derivatives containing BODIPY FL and NBD groups could be used to screen the binding of novel antibiotics to bacterial ribosomes in the macrolide-binding site.
作者简介
A. Tereshchenkov
Faculty of Chemistry
Email: sumbtyan@belozersky.msu.ru
俄罗斯联邦, Moscow, 119991
A. Shishkina
Belozersky Institute of Physico-Chemical Biology
Email: sumbtyan@belozersky.msu.ru
俄罗斯联邦, Moscow, 119991
V. Karpenko
Faculty of Chemistry
Email: sumbtyan@belozersky.msu.ru
俄罗斯联邦, Moscow, 119991
V. Chertkov
Faculty of Chemistry
Email: sumbtyan@belozersky.msu.ru
俄罗斯联邦, Moscow, 119991
A. Konevega
Molecular and Radiation Biophysics Division, Konstantinov Petersburg Nuclear Physics Institute; Peter the Great Saint-Petersburg Polytechnic University
Email: sumbtyan@belozersky.msu.ru
俄罗斯联邦, Gatchina, 188300; St. Petersburg, 195251
P. Kasatsky
Molecular and Radiation Biophysics Division, Konstantinov Petersburg Nuclear Physics Institute; Peter the Great Saint-Petersburg Polytechnic University
Email: sumbtyan@belozersky.msu.ru
俄罗斯联邦, Gatchina, 188300; St. Petersburg, 195251
A. Bogdanov
Faculty of Chemistry; Belozersky Institute of Physico-Chemical Biology
Email: sumbtyan@belozersky.msu.ru
俄罗斯联邦, Moscow, 119991; Moscow, 119991
N. Sumbatyan
Faculty of Chemistry
编辑信件的主要联系方式.
Email: sumbtyan@belozersky.msu.ru
俄罗斯联邦, Moscow, 119991
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