New fluorescent macrolide derivatives for studying interactions of antibiotics and their analogs with the ribosomal exit tunnel
- Авторы: Tereshchenkov A.1, Shishkina A.2, Karpenko V.1, Chertkov V.1, Konevega A.3,4, Kasatsky P.3,4, Bogdanov A.1,2, Sumbatyan N.1
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Учреждения:
- Faculty of Chemistry
- Belozersky Institute of Physico-Chemical Biology
- Molecular and Radiation Biophysics Division, Konstantinov Petersburg Nuclear Physics Institute
- Peter the Great Saint-Petersburg Polytechnic University
- Выпуск: Том 81, № 10 (2016)
- Страницы: 1163-1172
- Раздел: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151045
- DOI: https://doi.org/10.1134/S0006297916100138
- ID: 151045
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Аннотация
Novel fluorescent derivatives of macrolide antibiotics related to tylosin bearing rhodamine, fluorescein, Alexa Fluor 488, BODIPY FL, and nitrobenzoxadiazole (NBD) residues were synthesized. The formation of complexes of these compounds with 70S E. coli ribosomes was studied by measuring the fluorescence polarization depending on the ribosome amount at constant concentration of the fluorescent substance. With the synthesized fluorescent tylosin derivatives, the dissociation constants for ribosome complexes with several known antibiotics and macrolide analogs previously obtained were determined. It was found that the fluorescent tylosin derivatives containing BODIPY FL and NBD groups could be used to screen the binding of novel antibiotics to bacterial ribosomes in the macrolide-binding site.
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Об авторах
A. Tereshchenkov
Faculty of Chemistry
Email: sumbtyan@belozersky.msu.ru
Россия, Moscow, 119991
A. Shishkina
Belozersky Institute of Physico-Chemical Biology
Email: sumbtyan@belozersky.msu.ru
Россия, Moscow, 119991
V. Karpenko
Faculty of Chemistry
Email: sumbtyan@belozersky.msu.ru
Россия, Moscow, 119991
V. Chertkov
Faculty of Chemistry
Email: sumbtyan@belozersky.msu.ru
Россия, Moscow, 119991
A. Konevega
Molecular and Radiation Biophysics Division, Konstantinov Petersburg Nuclear Physics Institute; Peter the Great Saint-Petersburg Polytechnic University
Email: sumbtyan@belozersky.msu.ru
Россия, Gatchina, 188300; St. Petersburg, 195251
P. Kasatsky
Molecular and Radiation Biophysics Division, Konstantinov Petersburg Nuclear Physics Institute; Peter the Great Saint-Petersburg Polytechnic University
Email: sumbtyan@belozersky.msu.ru
Россия, Gatchina, 188300; St. Petersburg, 195251
A. Bogdanov
Faculty of Chemistry; Belozersky Institute of Physico-Chemical Biology
Email: sumbtyan@belozersky.msu.ru
Россия, Moscow, 119991; Moscow, 119991
N. Sumbatyan
Faculty of Chemistry
Автор, ответственный за переписку.
Email: sumbtyan@belozersky.msu.ru
Россия, Moscow, 119991
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