New fluorescent macrolide derivatives for studying interactions of antibiotics and their analogs with the ribosomal exit tunnel
- Authors: Tereshchenkov A.G.1, Shishkina A.V.2, Karpenko V.V.1, Chertkov V.A.1, Konevega A.L.3,4, Kasatsky P.S.3,4, Bogdanov A.A.1,2, Sumbatyan N.V.1
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Affiliations:
- Faculty of Chemistry
- Belozersky Institute of Physico-Chemical Biology
- Molecular and Radiation Biophysics Division, Konstantinov Petersburg Nuclear Physics Institute
- Peter the Great Saint-Petersburg Polytechnic University
- Issue: Vol 81, No 10 (2016)
- Pages: 1163-1172
- Section: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151045
- DOI: https://doi.org/10.1134/S0006297916100138
- ID: 151045
Cite item
Abstract
Novel fluorescent derivatives of macrolide antibiotics related to tylosin bearing rhodamine, fluorescein, Alexa Fluor 488, BODIPY FL, and nitrobenzoxadiazole (NBD) residues were synthesized. The formation of complexes of these compounds with 70S E. coli ribosomes was studied by measuring the fluorescence polarization depending on the ribosome amount at constant concentration of the fluorescent substance. With the synthesized fluorescent tylosin derivatives, the dissociation constants for ribosome complexes with several known antibiotics and macrolide analogs previously obtained were determined. It was found that the fluorescent tylosin derivatives containing BODIPY FL and NBD groups could be used to screen the binding of novel antibiotics to bacterial ribosomes in the macrolide-binding site.
About the authors
A. G. Tereshchenkov
Faculty of Chemistry
Email: sumbtyan@belozersky.msu.ru
Russian Federation, Moscow, 119991
A. V. Shishkina
Belozersky Institute of Physico-Chemical Biology
Email: sumbtyan@belozersky.msu.ru
Russian Federation, Moscow, 119991
V. V. Karpenko
Faculty of Chemistry
Email: sumbtyan@belozersky.msu.ru
Russian Federation, Moscow, 119991
V. A. Chertkov
Faculty of Chemistry
Email: sumbtyan@belozersky.msu.ru
Russian Federation, Moscow, 119991
A. L. Konevega
Molecular and Radiation Biophysics Division, Konstantinov Petersburg Nuclear Physics Institute; Peter the Great Saint-Petersburg Polytechnic University
Email: sumbtyan@belozersky.msu.ru
Russian Federation, Gatchina, 188300; St. Petersburg, 195251
P. S. Kasatsky
Molecular and Radiation Biophysics Division, Konstantinov Petersburg Nuclear Physics Institute; Peter the Great Saint-Petersburg Polytechnic University
Email: sumbtyan@belozersky.msu.ru
Russian Federation, Gatchina, 188300; St. Petersburg, 195251
A. A. Bogdanov
Faculty of Chemistry; Belozersky Institute of Physico-Chemical Biology
Email: sumbtyan@belozersky.msu.ru
Russian Federation, Moscow, 119991; Moscow, 119991
N. V. Sumbatyan
Faculty of Chemistry
Author for correspondence.
Email: sumbtyan@belozersky.msu.ru
Russian Federation, Moscow, 119991