Factors beyond enolase 2 and mitochondrial lysyl-tRNA synthetase precursor are required for tRNA import into yeast mitochondria
- Авторы: Baleva M.1,2, Meyer M.3, Entelis N.1, Tarassov I.1, Kamenski P.2,4, Masquida B.1
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Учреждения:
- GMGM
- Lomonosov Moscow State University
- IGBMC
- Immanuil Kant Baltic Federal University
- Выпуск: Том 82, № 11 (2017)
- Страницы: 1324-1335
- Раздел: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151510
- DOI: https://doi.org/10.1134/S0006297917110104
- ID: 151510
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Аннотация
In yeast, the import of tRNALys with CUU anticodon (tRK1) relies on a complex mechanism where interaction with enolase 2 (Eno2p) dictates a deep conformational change of the tRNA. This event is believed to mask the tRNA from the cytosolic translational machinery to re-direct it towards the mitochondria. Once near the mitochondrial outer membrane, the precursor of the mitochondrial lysyl-tRNA synthetase (preMsk1p) takes over enolase to carry the tRNA within the mitochondrial matrix, where it is supposed to participate in translation following correct refolding. Biochemical data presented in this report focus on the role of enolase. They show that despite the inability of Eno2p alone to form a complex with tRK1, mitochondrial import can be recapitulated in vitro using fractions of yeast extracts sharing either recombinant or endogenous yeast Eno2p as one of the main components. Taken together, our data suggest the existence of a protein complex containing Eno2p that is involved in RNA mitochondrial import.
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Об авторах
M. Baleva
GMGM; Lomonosov Moscow State University
Email: b.masquida@unistra.fr
Франция, UMR 7156, Strasbourg, 67081; Moscow, 119991
M. Meyer
IGBMC
Email: b.masquida@unistra.fr
Франция, UMR 7104, Illkirch, 67404
N. Entelis
GMGM
Email: b.masquida@unistra.fr
Франция, UMR 7156, Strasbourg, 67081
I. Tarassov
GMGM
Email: b.masquida@unistra.fr
Франция, UMR 7156, Strasbourg, 67081
P. Kamenski
Lomonosov Moscow State University; Immanuil Kant Baltic Federal University
Email: b.masquida@unistra.fr
Россия, Moscow, 119991; Kaliningrad, 236038
B. Masquida
GMGM
Автор, ответственный за переписку.
Email: b.masquida@unistra.fr
Франция, UMR 7156, Strasbourg, 67081
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