Factors beyond enolase 2 and mitochondrial lysyl-tRNA synthetase precursor are required for tRNA import into yeast mitochondria
- 作者: Baleva M.1,2, Meyer M.3, Entelis N.1, Tarassov I.1, Kamenski P.2,4, Masquida B.1
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隶属关系:
- GMGM
- Lomonosov Moscow State University
- IGBMC
- Immanuil Kant Baltic Federal University
- 期: 卷 82, 编号 11 (2017)
- 页面: 1324-1335
- 栏目: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151510
- DOI: https://doi.org/10.1134/S0006297917110104
- ID: 151510
如何引用文章
详细
In yeast, the import of tRNALys with CUU anticodon (tRK1) relies on a complex mechanism where interaction with enolase 2 (Eno2p) dictates a deep conformational change of the tRNA. This event is believed to mask the tRNA from the cytosolic translational machinery to re-direct it towards the mitochondria. Once near the mitochondrial outer membrane, the precursor of the mitochondrial lysyl-tRNA synthetase (preMsk1p) takes over enolase to carry the tRNA within the mitochondrial matrix, where it is supposed to participate in translation following correct refolding. Biochemical data presented in this report focus on the role of enolase. They show that despite the inability of Eno2p alone to form a complex with tRK1, mitochondrial import can be recapitulated in vitro using fractions of yeast extracts sharing either recombinant or endogenous yeast Eno2p as one of the main components. Taken together, our data suggest the existence of a protein complex containing Eno2p that is involved in RNA mitochondrial import.
作者简介
M. Baleva
GMGM; Lomonosov Moscow State University
Email: b.masquida@unistra.fr
法国, UMR 7156, Strasbourg, 67081; Moscow, 119991
M. Meyer
IGBMC
Email: b.masquida@unistra.fr
法国, UMR 7104, Illkirch, 67404
N. Entelis
GMGM
Email: b.masquida@unistra.fr
法国, UMR 7156, Strasbourg, 67081
I. Tarassov
GMGM
Email: b.masquida@unistra.fr
法国, UMR 7156, Strasbourg, 67081
P. Kamenski
Lomonosov Moscow State University; Immanuil Kant Baltic Federal University
Email: b.masquida@unistra.fr
俄罗斯联邦, Moscow, 119991; Kaliningrad, 236038
B. Masquida
GMGM
编辑信件的主要联系方式.
Email: b.masquida@unistra.fr
法国, UMR 7156, Strasbourg, 67081
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