Enviar artigo por via de e-mail Laccase-Catalyzed Heterocoupling of Dihydroquercetin and p-Aminobenzoic Acid: Effect of the Reaction Product on Cultured Cells
- Autores: Khlupova M.1, Morozova O.1, Vasil’eva I.1, Shumakovich G.1, Pashintseva N.1, Kovalev L.1, Shishkin S.1, Chertkov V.2, Shestakova A.3, Kisin A.3, Yaropolov A.1
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Afiliações:
- Research Center of Biotechnology, Bach Institute of Biochemistry
- Lomonosov Moscow State University, Faculty of Chemistry
- State Research Institute of Chemistry and Technology of Organoelement Compounds
- Edição: Volume 83, Nº 8 (2018)
- Páginas: 992-1001
- Seção: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151704
- DOI: https://doi.org/10.1134/S0006297918080102
- ID: 151704
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Resumo
Derivatization of the natural flavonoid dihydroquercetin with p-aminobenzoic acid was carried out in an ethyl acetate/citric buffer biphasic system using laccase from the fungus Trametes hirsuta. The main reaction product yield was ~68 mol %. The product was characterized by 1H NMR, 13C NMR, and liquid chromatography-mass spectroscopy, and its structure was elucidated. The reaction product affected viability of cultured human rhabdomyosarcoma cells (RD cell line) in a dose-dependent manner and, therefore, can be of interest to pharmaceutical industry.
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Sobre autores
M. Khlupova
Research Center of Biotechnology, Bach Institute of Biochemistry
Email: yaropolov@inbi.ras.ru
Rússia, Moscow, 119071
O. Morozova
Research Center of Biotechnology, Bach Institute of Biochemistry
Email: yaropolov@inbi.ras.ru
Rússia, Moscow, 119071
I. Vasil’eva
Research Center of Biotechnology, Bach Institute of Biochemistry
Email: yaropolov@inbi.ras.ru
Rússia, Moscow, 119071
G. Shumakovich
Research Center of Biotechnology, Bach Institute of Biochemistry
Email: yaropolov@inbi.ras.ru
Rússia, Moscow, 119071
N. Pashintseva
Research Center of Biotechnology, Bach Institute of Biochemistry
Email: yaropolov@inbi.ras.ru
Rússia, Moscow, 119071
L. Kovalev
Research Center of Biotechnology, Bach Institute of Biochemistry
Email: yaropolov@inbi.ras.ru
Rússia, Moscow, 119071
S. Shishkin
Research Center of Biotechnology, Bach Institute of Biochemistry
Email: yaropolov@inbi.ras.ru
Rússia, Moscow, 119071
V. Chertkov
Lomonosov Moscow State University, Faculty of Chemistry
Email: yaropolov@inbi.ras.ru
Rússia, Moscow, 119899
A. Shestakova
State Research Institute of Chemistry and Technology of Organoelement Compounds
Email: yaropolov@inbi.ras.ru
Rússia, Moscow, 105118
A. Kisin
State Research Institute of Chemistry and Technology of Organoelement Compounds
Email: yaropolov@inbi.ras.ru
Rússia, Moscow, 105118
A. Yaropolov
Research Center of Biotechnology, Bach Institute of Biochemistry
Autor responsável pela correspondência
Email: yaropolov@inbi.ras.ru
Rússia, Moscow, 119071
