Email this article Laccase-Catalyzed Heterocoupling of Dihydroquercetin and p-Aminobenzoic Acid: Effect of the Reaction Product on Cultured Cells
- Authors: Khlupova M.E.1, Morozova O.V.1, Vasil’eva I.S.1, Shumakovich G.P.1, Pashintseva N.V.1, Kovalev L.I.1, Shishkin S.S.1, Chertkov V.A.2, Shestakova A.K.3, Kisin A.V.3, Yaropolov A.I.1
-
Affiliations:
- Research Center of Biotechnology, Bach Institute of Biochemistry
- Lomonosov Moscow State University, Faculty of Chemistry
- State Research Institute of Chemistry and Technology of Organoelement Compounds
- Issue: Vol 83, No 8 (2018)
- Pages: 992-1001
- Section: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151704
- DOI: https://doi.org/10.1134/S0006297918080102
- ID: 151704
Cite item
Open Access
Access granted
Subscription Access
Abstract
Derivatization of the natural flavonoid dihydroquercetin with p-aminobenzoic acid was carried out in an ethyl acetate/citric buffer biphasic system using laccase from the fungus Trametes hirsuta. The main reaction product yield was ~68 mol %. The product was characterized by 1H NMR, 13C NMR, and liquid chromatography-mass spectroscopy, and its structure was elucidated. The reaction product affected viability of cultured human rhabdomyosarcoma cells (RD cell line) in a dose-dependent manner and, therefore, can be of interest to pharmaceutical industry.
About the authors
M. E. Khlupova
Research Center of Biotechnology, Bach Institute of Biochemistry
Email: yaropolov@inbi.ras.ru
Russian Federation, Moscow, 119071
O. V. Morozova
Research Center of Biotechnology, Bach Institute of Biochemistry
Email: yaropolov@inbi.ras.ru
Russian Federation, Moscow, 119071
I. S. Vasil’eva
Research Center of Biotechnology, Bach Institute of Biochemistry
Email: yaropolov@inbi.ras.ru
Russian Federation, Moscow, 119071
G. P. Shumakovich
Research Center of Biotechnology, Bach Institute of Biochemistry
Email: yaropolov@inbi.ras.ru
Russian Federation, Moscow, 119071
N. V. Pashintseva
Research Center of Biotechnology, Bach Institute of Biochemistry
Email: yaropolov@inbi.ras.ru
Russian Federation, Moscow, 119071
L. I. Kovalev
Research Center of Biotechnology, Bach Institute of Biochemistry
Email: yaropolov@inbi.ras.ru
Russian Federation, Moscow, 119071
S. S. Shishkin
Research Center of Biotechnology, Bach Institute of Biochemistry
Email: yaropolov@inbi.ras.ru
Russian Federation, Moscow, 119071
V. A. Chertkov
Lomonosov Moscow State University, Faculty of Chemistry
Email: yaropolov@inbi.ras.ru
Russian Federation, Moscow, 119899
A. K. Shestakova
State Research Institute of Chemistry and Technology of Organoelement Compounds
Email: yaropolov@inbi.ras.ru
Russian Federation, Moscow, 105118
A. V. Kisin
State Research Institute of Chemistry and Technology of Organoelement Compounds
Email: yaropolov@inbi.ras.ru
Russian Federation, Moscow, 105118
A. I. Yaropolov
Research Center of Biotechnology, Bach Institute of Biochemistry
Author for correspondence.
Email: yaropolov@inbi.ras.ru
Russian Federation, Moscow, 119071
