Effect of low temperature on globin expression, respiratory metabolic enzyme activities, and gill structure of  Litopenaeus vannamei

Meng Wu; Nan Chen; Chun-Xiao Huang; Yan He; Yong-Zhen Zhao; Xiao-Han Chen; Xiu-Li Chen; Huan-Ling Wang

doi:10.1134/S0006297917070100

Effect of low temperature on globin expression, respiratory metabolic enzyme activities, and gill structure of Litopenaeus vannamei

Autores: Wu M.¹, Chen N.¹, Huang C.¹, He Y.¹, Zhao Y.², Chen X.³, Chen X.³, Wang H.¹^,2
Afiliações:
1. Ministry of Education, Huazhong Agricultural University, College of Fishery, Key Lab of Freshwater Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction
2. Freshwater Aquaculture Collaborative Innovation Center of Hubei Province
3. Guangxi Academy of Fishery Sciences
Edição: Volume 82, Nº 7 (2017)
Páginas: 844-851
Seção: Article
URL: https://journals.rcsi.science/0006-2979/article/view/151441
DOI: https://doi.org/10.1134/S0006297917070100
ID: 151441

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Resumo

Low temperature frequently influences growth, development, and even survival of aquatic animals. In the present study, physiological and molecular responses to low temperature in Litopenaeus vannamei were investigated. The cDNA sequences of two oxygen-carrying proteins, cytoglobin (Cygb) and neuroglobin (Ngb), were isolated. Protein structure analysis revealed that both proteins share a globin superfamily domain. Real-time PCR analysis indicated that Cygb and Ngb mRNA levels gradually increased during decrease in temperatures from 25 to 15°C and then decreased at 10°C in muscle, brain, stomach, and heart, except for a continuing increase in gills, whereas they showed a different expression trend in the hepatopancreas. Hemocyanin concentration gradually reduced as the temperature decreased. Moreover, the activities of respiratory metabolic enzymes including lactate dehydrogenase (LDH) and succinate dehydrogenase (SDH) were measured, and it was found that LDH activity gradually increased while SDH activity decreased after low-temperature treatment. Finally, damage to gill structure at low temperature was also observed, and this intensified with further decrease in temperature. Taken together, these results show that low temperature has an adverse influence in L. vannamei, which contributes to systematic understanding of the adaptation mechanisms of shrimp at low temperature.

Palavras-chave

Litopenaeus vannamei, low temperature, Cygb, Ngb, respiratory enzymes, gill structure

Sobre autores

Meng Wu

Ministry of Education, Huazhong Agricultural University, College of Fishery, Key Lab of Freshwater Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction