Email this article Effect of low temperature on globin expression, respiratory metabolic enzyme activities, and gill structure of Litopenaeus vannamei
- Authors: Wu M.1, Chen N.1, Huang C.1, He Y.1, Zhao Y.2, Chen X.3, Chen X.3, Wang H.1,2
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Affiliations:
- Ministry of Education, Huazhong Agricultural University, College of Fishery, Key Lab of Freshwater Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction
- Freshwater Aquaculture Collaborative Innovation Center of Hubei Province
- Guangxi Academy of Fishery Sciences
- Issue: Vol 82, No 7 (2017)
- Pages: 844-851
- Section: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151441
- DOI: https://doi.org/10.1134/S0006297917070100
- ID: 151441
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Abstract
Low temperature frequently influences growth, development, and even survival of aquatic animals. In the present study, physiological and molecular responses to low temperature in Litopenaeus vannamei were investigated. The cDNA sequences of two oxygen-carrying proteins, cytoglobin (Cygb) and neuroglobin (Ngb), were isolated. Protein structure analysis revealed that both proteins share a globin superfamily domain. Real-time PCR analysis indicated that Cygb and Ngb mRNA levels gradually increased during decrease in temperatures from 25 to 15°C and then decreased at 10°C in muscle, brain, stomach, and heart, except for a continuing increase in gills, whereas they showed a different expression trend in the hepatopancreas. Hemocyanin concentration gradually reduced as the temperature decreased. Moreover, the activities of respiratory metabolic enzymes including lactate dehydrogenase (LDH) and succinate dehydrogenase (SDH) were measured, and it was found that LDH activity gradually increased while SDH activity decreased after low-temperature treatment. Finally, damage to gill structure at low temperature was also observed, and this intensified with further decrease in temperature. Taken together, these results show that low temperature has an adverse influence in L. vannamei, which contributes to systematic understanding of the adaptation mechanisms of shrimp at low temperature.
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About the authors
Meng Wu
Ministry of Education, Huazhong Agricultural University, College of Fishery, Key Lab of Freshwater Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction
Email: hbauwhl@hotmail.com
China, Wuhan, 430070
Nan Chen
Ministry of Education, Huazhong Agricultural University, College of Fishery, Key Lab of Freshwater Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction
Email: hbauwhl@hotmail.com
China, Wuhan, 430070
Chun-Xiao Huang
Ministry of Education, Huazhong Agricultural University, College of Fishery, Key Lab of Freshwater Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction
Email: hbauwhl@hotmail.com
China, Wuhan, 430070
Yan He
Ministry of Education, Huazhong Agricultural University, College of Fishery, Key Lab of Freshwater Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction
Email: hbauwhl@hotmail.com
China, Wuhan, 430070
Yong-Zhen Zhao
Freshwater Aquaculture Collaborative Innovation Center of Hubei Province
Email: hbauwhl@hotmail.com
China, Wuhan, 430070
Xiao-Han Chen
Guangxi Academy of Fishery Sciences
Email: hbauwhl@hotmail.com
China, Nanning, 530021
Xiu-Li Chen
Guangxi Academy of Fishery Sciences
Author for correspondence.
Email: chenxiuli2001@163.com
China, Nanning, 530021
Huan-Ling Wang
Ministry of Education, Huazhong Agricultural University, College of Fishery, Key Lab of Freshwater Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province
Author for correspondence.
Email: hbauwhl@hotmail.com
China, Wuhan, 430070; Wuhan, 430070
