Cell wall glycopolymers of type strains from three species of the genus Actinoplanes
- Autores: Streshinskaya G.1, Sashkov A.2, Tul’skaya E.1, Senchenkova S.2, Dmitrenok A.2, Piskunkova N.1, Bueva O.3, Evtushenko L.3
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Afiliações:
- Lomonosov Moscow State University
- Zelinsky Institute of Organic Chemistry
- All-Russian Collection of Microorganisms, Skryabin Institute of Biochemistry and Physiology of Microorganisms
- Edição: Volume 81, Nº 9 (2016)
- Páginas: 999-1012
- Seção: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151010
- DOI: https://doi.org/10.1134/S0006297916090091
- ID: 151010
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Resumo
The structures of cell wall glycopolymers from the type strains of three Actinoplanes species were investigated using chemical methods, NMR spectroscopy, and mass spectrometry. Actinoplanes digitatis VKM Ac-649T contains two phosphate-containing glycopolymers: poly(diglycosyl-1-phosphate) →6)-α-D-GlcpNAc-(1-P-6)-α-D-GlcpN-(1→ and teichoic acid →1)-sn-Gro-(3-P-3)-β-[β-D-GlcpNAc-(1→2]-D-Galp-(1→. Two glycopolymers were identified in A. auranticolor VKM Ac-648T and A. cyaneus VKM Ac-1095T: minor polymer–unsubstituted 2,3-poly(glycerol phosphate), widely abundant in actinobacteria (Ac-648T), and mannan with trisaccharide repeating unit →2)-α-D-Manp-(1→2)-α-D-Manp(1→6)-α-D-Manp-(1→(Ac-1095T). In addition, both microorganisms contain a teichuronic acid of unique structure containing a pentasaccharide repeating unit with two residues of glucopyranose and three residues of diaminouronic acids in D-manno- and/or D-gluco-configuration. Each of the strains demonstrates peculiarities in the structure of teichuronic acid with respect to the ratio of diaminouronic acids and availability and location of O-methyl groups in glucopyranose residues. All investigated strains contain a unique set of glycopolymers in their cell walls with structures not described earlier for prokaryotes.
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Sobre autores
G. Streshinskaya
Lomonosov Moscow State University
Autor responsável pela correspondência
Email: streshinskaya@mail.ru
Rússia, Moscow, 119991
A. Sashkov
Zelinsky Institute of Organic Chemistry
Email: streshinskaya@mail.ru
Rússia, Moscow, 119991
E. Tul’skaya
Lomonosov Moscow State University
Email: streshinskaya@mail.ru
Rússia, Moscow, 119991
S. Senchenkova
Zelinsky Institute of Organic Chemistry
Email: streshinskaya@mail.ru
Rússia, Moscow, 119991
A. Dmitrenok
Zelinsky Institute of Organic Chemistry
Email: streshinskaya@mail.ru
Rússia, Moscow, 119991
N. Piskunkova
Lomonosov Moscow State University
Email: streshinskaya@mail.ru
Rússia, Moscow, 119991
O. Bueva
All-Russian Collection of Microorganisms, Skryabin Institute of Biochemistry and Physiology of Microorganisms
Email: streshinskaya@mail.ru
Rússia, Pushchino, Moscow Region, 142290
L. Evtushenko
All-Russian Collection of Microorganisms, Skryabin Institute of Biochemistry and Physiology of Microorganisms
Email: streshinskaya@mail.ru
Rússia, Pushchino, Moscow Region, 142290
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