Cell wall glycopolymers of type strains from three species of the genus Actinoplanes
- Авторы: Streshinskaya G.1, Sashkov A.2, Tul’skaya E.1, Senchenkova S.2, Dmitrenok A.2, Piskunkova N.1, Bueva O.3, Evtushenko L.3
-
Учреждения:
- Lomonosov Moscow State University
- Zelinsky Institute of Organic Chemistry
- All-Russian Collection of Microorganisms, Skryabin Institute of Biochemistry and Physiology of Microorganisms
- Выпуск: Том 81, № 9 (2016)
- Страницы: 999-1012
- Раздел: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151010
- DOI: https://doi.org/10.1134/S0006297916090091
- ID: 151010
Цитировать
Аннотация
The structures of cell wall glycopolymers from the type strains of three Actinoplanes species were investigated using chemical methods, NMR spectroscopy, and mass spectrometry. Actinoplanes digitatis VKM Ac-649T contains two phosphate-containing glycopolymers: poly(diglycosyl-1-phosphate) →6)-α-D-GlcpNAc-(1-P-6)-α-D-GlcpN-(1→ and teichoic acid →1)-sn-Gro-(3-P-3)-β-[β-D-GlcpNAc-(1→2]-D-Galp-(1→. Two glycopolymers were identified in A. auranticolor VKM Ac-648T and A. cyaneus VKM Ac-1095T: minor polymer–unsubstituted 2,3-poly(glycerol phosphate), widely abundant in actinobacteria (Ac-648T), and mannan with trisaccharide repeating unit →2)-α-D-Manp-(1→2)-α-D-Manp(1→6)-α-D-Manp-(1→(Ac-1095T). In addition, both microorganisms contain a teichuronic acid of unique structure containing a pentasaccharide repeating unit with two residues of glucopyranose and three residues of diaminouronic acids in D-manno- and/or D-gluco-configuration. Each of the strains demonstrates peculiarities in the structure of teichuronic acid with respect to the ratio of diaminouronic acids and availability and location of O-methyl groups in glucopyranose residues. All investigated strains contain a unique set of glycopolymers in their cell walls with structures not described earlier for prokaryotes.
Ключевые слова
Об авторах
G. Streshinskaya
Lomonosov Moscow State University
Автор, ответственный за переписку.
Email: streshinskaya@mail.ru
Россия, Moscow, 119991
A. Sashkov
Zelinsky Institute of Organic Chemistry
Email: streshinskaya@mail.ru
Россия, Moscow, 119991
E. Tul’skaya
Lomonosov Moscow State University
Email: streshinskaya@mail.ru
Россия, Moscow, 119991
S. Senchenkova
Zelinsky Institute of Organic Chemistry
Email: streshinskaya@mail.ru
Россия, Moscow, 119991
A. Dmitrenok
Zelinsky Institute of Organic Chemistry
Email: streshinskaya@mail.ru
Россия, Moscow, 119991
N. Piskunkova
Lomonosov Moscow State University
Email: streshinskaya@mail.ru
Россия, Moscow, 119991
O. Bueva
All-Russian Collection of Microorganisms, Skryabin Institute of Biochemistry and Physiology of Microorganisms
Email: streshinskaya@mail.ru
Россия, Pushchino, Moscow Region, 142290
L. Evtushenko
All-Russian Collection of Microorganisms, Skryabin Institute of Biochemistry and Physiology of Microorganisms
Email: streshinskaya@mail.ru
Россия, Pushchino, Moscow Region, 142290
![](/img/style/loading.gif)