Heterologous expression of chaperones from hyperthermophilic archaea inhibits aminoglycoside-induced protein misfolding in Escherichia coli
- Authors: Peng S.1, Chu Z.2,3, Lu J.2,3, Li D.2,3, Wang Y.1, Yang S.2,3, Zhang Y.2,3
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Affiliations:
- State Key Laboratory of Bioreactor Engineering
- Shanghai Institutes for Biological Sciences
- Key Laboratory of Synthetic Biology
- Issue: Vol 82, No 10 (2017)
- Pages: 1169-1175
- Section: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151485
- DOI: https://doi.org/10.1134/S0006297917100091
- ID: 151485
Cite item
Abstract
Aminoglycoside antibiotics affect protein translation fidelity and lead to protein aggregation and an increase in intracellular oxidative stress level as well. The overexpression of the chaperonin GroEL/GroES system promotes short-term tolerance to aminoglycosides in Escherichia coli. Here, we demonstrated that the coexpression of prefoldin or Hsp60 originating from the hyperthermophilic archaeon Pyrococcus furiosus in E. coli cells can rescue cell growth and inhibit protein aggregation induced by streptomycin exposure. The results of our study show that hyperthermophilic chaperones endow E. coli with a higher tolerance to streptomycin than the GroEL/GroES system, and that they exert better effects on the reduction of intracellular protein misfolding, indicating that these chaperones have unique features and functions.
About the authors
S. Peng
State Key Laboratory of Bioreactor Engineering
Email: yzhang@sibs.ac.cn
China, Shanghai, 200237
Z. Chu
Shanghai Institutes for Biological Sciences; Key Laboratory of Synthetic Biology
Email: yzhang@sibs.ac.cn
China, Shanghai, 200031; Shanghai, 200032
J. Lu
Shanghai Institutes for Biological Sciences; Key Laboratory of Synthetic Biology
Email: yzhang@sibs.ac.cn
China, Shanghai, 200031; Shanghai, 200032
D. Li
Shanghai Institutes for Biological Sciences; Key Laboratory of Synthetic Biology
Email: yzhang@sibs.ac.cn
China, Shanghai, 200031; Shanghai, 200032
Y. Wang
State Key Laboratory of Bioreactor Engineering
Author for correspondence.
Email: yhwang@ecust.edu.cn
China, Shanghai, 200237
S. Yang
Shanghai Institutes for Biological Sciences; Key Laboratory of Synthetic Biology
Email: yzhang@sibs.ac.cn
China, Shanghai, 200031; Shanghai, 200032
Y. Zhang
Shanghai Institutes for Biological Sciences; Key Laboratory of Synthetic Biology
Author for correspondence.
Email: yzhang@sibs.ac.cn
China, Shanghai, 200031; Shanghai, 200032
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