Heterologous expression of chaperones from hyperthermophilic archaea inhibits aminoglycoside-induced protein misfolding in Escherichia coli
- 作者: Peng S.1, Chu Z.2,3, Lu J.2,3, Li D.2,3, Wang Y.1, Yang S.2,3, Zhang Y.2,3
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隶属关系:
- State Key Laboratory of Bioreactor Engineering
- Shanghai Institutes for Biological Sciences
- Key Laboratory of Synthetic Biology
- 期: 卷 82, 编号 10 (2017)
- 页面: 1169-1175
- 栏目: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151485
- DOI: https://doi.org/10.1134/S0006297917100091
- ID: 151485
如何引用文章
详细
Aminoglycoside antibiotics affect protein translation fidelity and lead to protein aggregation and an increase in intracellular oxidative stress level as well. The overexpression of the chaperonin GroEL/GroES system promotes short-term tolerance to aminoglycosides in Escherichia coli. Here, we demonstrated that the coexpression of prefoldin or Hsp60 originating from the hyperthermophilic archaeon Pyrococcus furiosus in E. coli cells can rescue cell growth and inhibit protein aggregation induced by streptomycin exposure. The results of our study show that hyperthermophilic chaperones endow E. coli with a higher tolerance to streptomycin than the GroEL/GroES system, and that they exert better effects on the reduction of intracellular protein misfolding, indicating that these chaperones have unique features and functions.
作者简介
S. Peng
State Key Laboratory of Bioreactor Engineering
Email: yzhang@sibs.ac.cn
中国, Shanghai, 200237
Z. Chu
Shanghai Institutes for Biological Sciences; Key Laboratory of Synthetic Biology
Email: yzhang@sibs.ac.cn
中国, Shanghai, 200031; Shanghai, 200032
J. Lu
Shanghai Institutes for Biological Sciences; Key Laboratory of Synthetic Biology
Email: yzhang@sibs.ac.cn
中国, Shanghai, 200031; Shanghai, 200032
D. Li
Shanghai Institutes for Biological Sciences; Key Laboratory of Synthetic Biology
Email: yzhang@sibs.ac.cn
中国, Shanghai, 200031; Shanghai, 200032
Y. Wang
State Key Laboratory of Bioreactor Engineering
编辑信件的主要联系方式.
Email: yhwang@ecust.edu.cn
中国, Shanghai, 200237
S. Yang
Shanghai Institutes for Biological Sciences; Key Laboratory of Synthetic Biology
Email: yzhang@sibs.ac.cn
中国, Shanghai, 200031; Shanghai, 200032
Y. Zhang
Shanghai Institutes for Biological Sciences; Key Laboratory of Synthetic Biology
编辑信件的主要联系方式.
Email: yzhang@sibs.ac.cn
中国, Shanghai, 200031; Shanghai, 200032