Nigericin inhibits epithelial ovarian cancer metastasis by suppressing the cell cycle and epithelial−mesenchymal transition
- Authors: Wang W.1, Zhao Y.1, Yao S.1, Cui X.1, Pan W.1, Huang W.1, Gao J.2, Dong T.1, Zhang S.1
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Affiliations:
- Department of Obstetrics and Gynecology
- School of Life Science
- Issue: Vol 82, No 8 (2017)
- Pages: 933-941
- Section: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151453
- DOI: https://doi.org/10.1134/S0006297917080089
- ID: 151453
Cite item
Abstract
Epithelial ovarian cancer (EOC) has the highest mortality among various types of gynecological malignancies. Most patients die of metastasis and recurrence due to cisplatin resistance. Thus, it is urgent to develop novel therapies to cure this disease. CCK-8 assay showed that nigericin exhibited strong cytotoxicity on A2780 and SKOV3 cell lines. Flow cytometry indicated that nigericin could induce cell cycle arrest at G0/G1 phase and promote cell apoptosis. Boyden chamber assay revealed that nigericin could inhibit migration and invasion in a dose-dependent manner by suppressing epithelial–mesenchymal transition (EMT) in EOC cells. These effects were mediated, at least partly, by the Wnt/β-catenin signaling pathway. Our results demonstrated that nigericin could inhibit EMT during cell invasion and metastasis through the canonical Wnt/β-catenin signaling pathway. Nigericin may prove to be a novel therapeutic strategy that is effective in patients with metastatic EOC.
About the authors
Wen Wang
Department of Obstetrics and Gynecology; Department of Obstetrics and Gynecology
Email: info@pleiadesonline.com
China, Jinan, Shandong, 250012; Tengzhou, Shandong, 277500
Yan Zhao
Department of Obstetrics and Gynecology; Department of Obstetrics and Gynecology
Email: info@pleiadesonline.com
China, Jinan, Shandong, 250012; Linyi, Shandong, 276000
Shujuan Yao
Department of Obstetrics and Gynecology
Email: info@pleiadesonline.com
China, Tengzhou, Shandong, 277500
Xiujuan Cui
Department of Obstetrics and Gynecology
Email: info@pleiadesonline.com
China, Tengzhou, Shandong, 277500
Wenying Pan
Department of Obstetrics and Gynecology; Department of Obstetrics and Gynecology
Email: info@pleiadesonline.com
China, Jinan, Shandong, 250012; Binzhou, Shandong
Wenqian Huang
Department of Obstetrics and Gynecology
Email: info@pleiadesonline.com
China, Jinan, Shandong, 250012
Jiangang Gao
School of Life Science
Email: info@pleiadesonline.com
China, Jinan, Shandong
Taotao Dong
Department of Obstetrics and Gynecology
Email: info@pleiadesonline.com
China, Jinan, Shandong, 250012
Shiqian Zhang
Department of Obstetrics and Gynecology
Email: info@pleiadesonline.com
China, Jinan, Shandong, 250012