O-Antigens of Escherichia coli Strains O81 and HS3-104 Are Structurally and Genetically Related, Except O-Antigen Glucosylation in E. coli HS3-104
- Authors: Zdorovenko E.L.1, Wang Y.2, Shashkov A.S.1, Chen T.2, Ovchinnikova O.G.1, Liu B.2, Golomidova A.K.3, Babenko V.V.4, Letarov A.V.3, Knirel Y.A.1
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Affiliations:
- Zelinsky Institute of Organic Chemistry
- TEDA Institute of Biological Sciences and Biotechnology
- Winogradsky Institute of Microbiology, Research Center of Biotechnology
- Federal Research and Clinical Centre of Physico-Chemical Medicine
- Issue: Vol 83, No 5 (2018)
- Pages: 534-541
- Section: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151653
- DOI: https://doi.org/10.1134/S0006297918050061
- ID: 151653
Cite item
Abstract
Glycerophosphate-containing O-specific polysaccharides (OPSs) were obtained by mild acidic degradation of lipopolysaccharides isolated from Escherichia coli type strain O81 and E. coli strain HS3-104 from horse feces. The structures of both OPSs and of the oligosaccharide derived from the strain O81 OPS by treatment with 48% HF were studied by monosaccharide analysis and one- and two-dimensional 1H- and 13C-NMR spectroscopy. Both OPSs had similar structures and differed only in the presence of a side-chain glucose residue in the strain HS3-104 OPS. The genes and the organization of the O-antigen biosynthesis gene cluster in both strains are almost identical with the exception of the gtr gene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome.
About the authors
E. L. Zdorovenko
Zelinsky Institute of Organic Chemistry
Email: yknirel@gmail.com
Russian Federation, Moscow, 119991
Y. Wang
TEDA Institute of Biological Sciences and Biotechnology
Email: yknirel@gmail.com
China, Tianjin, 300457
A. S. Shashkov
Zelinsky Institute of Organic Chemistry
Email: yknirel@gmail.com
Russian Federation, Moscow, 119991
T. Chen
TEDA Institute of Biological Sciences and Biotechnology
Email: yknirel@gmail.com
China, Tianjin, 300457
O. G. Ovchinnikova
Zelinsky Institute of Organic Chemistry
Email: yknirel@gmail.com
Russian Federation, Moscow, 119991
B. Liu
TEDA Institute of Biological Sciences and Biotechnology
Email: yknirel@gmail.com
China, Tianjin, 300457
A. K. Golomidova
Winogradsky Institute of Microbiology, Research Center of Biotechnology
Email: yknirel@gmail.com
Russian Federation, Moscow, 117312
V. V. Babenko
Federal Research and Clinical Centre of Physico-Chemical Medicine
Email: yknirel@gmail.com
Russian Federation, Moscow, 119435
A. V. Letarov
Winogradsky Institute of Microbiology, Research Center of Biotechnology
Email: yknirel@gmail.com
Russian Federation, Moscow, 117312
Y. A. Knirel
Zelinsky Institute of Organic Chemistry
Author for correspondence.
Email: yknirel@gmail.com
Russian Federation, Moscow, 119991