O-Antigens of Escherichia coli Strains O81 and HS3-104 Are Structurally and Genetically Related, Except O-Antigen Glucosylation in E. coli HS3-104
- Авторы: Zdorovenko E.1, Wang Y.2, Shashkov A.1, Chen T.2, Ovchinnikova O.1, Liu B.2, Golomidova A.3, Babenko V.4, Letarov A.3, Knirel Y.1
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Учреждения:
- Zelinsky Institute of Organic Chemistry
- TEDA Institute of Biological Sciences and Biotechnology
- Winogradsky Institute of Microbiology, Research Center of Biotechnology
- Federal Research and Clinical Centre of Physico-Chemical Medicine
- Выпуск: Том 83, № 5 (2018)
- Страницы: 534-541
- Раздел: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151653
- DOI: https://doi.org/10.1134/S0006297918050061
- ID: 151653
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Аннотация
Glycerophosphate-containing O-specific polysaccharides (OPSs) were obtained by mild acidic degradation of lipopolysaccharides isolated from Escherichia coli type strain O81 and E. coli strain HS3-104 from horse feces. The structures of both OPSs and of the oligosaccharide derived from the strain O81 OPS by treatment with 48% HF were studied by monosaccharide analysis and one- and two-dimensional 1H- and 13C-NMR spectroscopy. Both OPSs had similar structures and differed only in the presence of a side-chain glucose residue in the strain HS3-104 OPS. The genes and the organization of the O-antigen biosynthesis gene cluster in both strains are almost identical with the exception of the gtr gene cluster responsible for glucosylations in the strain HS3-104, which is located elsewhere in the genome.
Об авторах
E. Zdorovenko
Zelinsky Institute of Organic Chemistry
Email: yknirel@gmail.com
Россия, Moscow, 119991
Y. Wang
TEDA Institute of Biological Sciences and Biotechnology
Email: yknirel@gmail.com
КНР, Tianjin, 300457
A. Shashkov
Zelinsky Institute of Organic Chemistry
Email: yknirel@gmail.com
Россия, Moscow, 119991
T. Chen
TEDA Institute of Biological Sciences and Biotechnology
Email: yknirel@gmail.com
КНР, Tianjin, 300457
O. Ovchinnikova
Zelinsky Institute of Organic Chemistry
Email: yknirel@gmail.com
Россия, Moscow, 119991
B. Liu
TEDA Institute of Biological Sciences and Biotechnology
Email: yknirel@gmail.com
КНР, Tianjin, 300457
A. Golomidova
Winogradsky Institute of Microbiology, Research Center of Biotechnology
Email: yknirel@gmail.com
Россия, Moscow, 117312
V. Babenko
Federal Research and Clinical Centre of Physico-Chemical Medicine
Email: yknirel@gmail.com
Россия, Moscow, 119435
A. Letarov
Winogradsky Institute of Microbiology, Research Center of Biotechnology
Email: yknirel@gmail.com
Россия, Moscow, 117312
Y. Knirel
Zelinsky Institute of Organic Chemistry
Автор, ответственный за переписку.
Email: yknirel@gmail.com
Россия, Moscow, 119991