Quantitative analysis of L1-retrotransposons in Alzheimer’s disease and aging
- Авторы: Protasova M.S.1, Gusev F.E.1, Grigorenko A.P.1,2, Kuznetsova I.L.1, Rogaev E.I.1,2,3, Andreeva T.V.1
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Учреждения:
- Vavilov Institute of General Genetics
- Department of Psychiatry
- Faculty of Biology, Faculty of Bioengineering and Bioinformatics
- Выпуск: Том 82, № 8 (2017)
- Страницы: 962-971
- Раздел: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151460
- DOI: https://doi.org/10.1134/S0006297917080120
- ID: 151460
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Аннотация
LINE1 retrotransposons are members of a class of mobile genetic elements capable of retrotransposition in the genome via a process of reverse transcription. LINE1 repeats, integrating into different chromosomal loci, affect the activity of genes and cause different genomic mutations. Somatic variability of the human genome is linked to the activity of some subfamilies of LINE1, in particular, a high level of LINE1 retrotranspositions has been observed in brain tissues. However, the contribution of LINE1 to genomic variability during normal aging and in age-related neurodegenerative diseases is poorly understood. We conducted quantitative real-time PCR analysis of active subfamilies of LINE1 repeats (aL1) using genomic DNA extracted from brain specimens of Alzheimer’s disease (AD) patients and individuals without neuropsychiatric pathologies, as well as DNA extracted from blood specimens of individuals of different ages (healthy and AD subjects). Inter-individual quantitative variations of active families of aL1 repeats in the genome were observed. No significant age-dependent differences were identified. Likewise, no difference of aL1 copy number in brain and blood were indicated between AD patients and the aged-matched control group without dementia. These data imply that aging and the AD-associated neurodegenerative process are not the major factors contributing to the retrotransposition processes of active LINE1 repeats.
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Об авторах
M. Protasova
Vavilov Institute of General Genetics
Автор, ответственный за переписку.
Email: maria2100@list.ru
Россия, Moscow, 119991
F. Gusev
Vavilov Institute of General Genetics
Email: maria2100@list.ru
Россия, Moscow, 119991
A. Grigorenko
Vavilov Institute of General Genetics; Department of Psychiatry
Email: maria2100@list.ru
Россия, Moscow, 119991; 303 Belmont Street, Worcester, MA, 01604
I. Kuznetsova
Vavilov Institute of General Genetics
Email: maria2100@list.ru
Россия, Moscow, 119991
E. Rogaev
Vavilov Institute of General Genetics; Department of Psychiatry; Faculty of Biology, Faculty of Bioengineering and Bioinformatics
Email: maria2100@list.ru
Россия, Moscow, 119991; 303 Belmont Street, Worcester, MA, 01604; Moscow, 119234
T. Andreeva
Vavilov Institute of General Genetics
Email: maria2100@list.ru
Россия, Moscow, 119991
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