Inhibition of Escherichia coli inorganic pyrophosphatase by fructose-1-phosphate


Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Тек жазылушылар үшін

Аннотация

Pyrophosphate regulates vital cellular reactions, and its level in E. coli cells is under the ultimate control of inorganic pyrophosphatase. The mechanisms involved in the regulation of pyrophosphatase activity still need to be elucidated. The present study demonstrated that fructose-1-phosphate inhibits pyrophosphatase activity by a mechanism not involving competition with substrate for binding to the active site. The inhibition constant governing the binding of the inhibitor to the enzyme–substrate complex is 1.1 mM. Substitutions of Lys112, Lys115, Lys148, and Arg43 in the regulatory site completely or partially abolished the inhibition. Thus, Fru-1-P is a physiological inhibitor of pyrophosphatase that acts via a regulatory site in this enzyme.

Авторлар туралы

N. Vorobyeva

Faculty of Chemistry; Belozersky Institute of Physico-Chemical Biology

Хат алмасуға жауапты Автор.
Email: nvorob@yandex.ru
Ресей, Moscow, 119991; Moscow, 119991

S. Kurilova

Belozersky Institute of Physico-Chemical Biology

Email: nvorob@yandex.ru
Ресей, Moscow, 119991

V. Anashkin

Belozersky Institute of Physico-Chemical Biology

Email: nvorob@yandex.ru
Ресей, Moscow, 119991

E. Rodina

Faculty of Chemistry

Email: nvorob@yandex.ru
Ресей, Moscow, 119991

Қосымша файлдар

Қосымша файлдар
Әрекет
1. JATS XML

© Pleiades Publishing, Ltd., 2017