Effects of fibroin microcarriers on inflammation and regeneration of deep skin wounds in mice

A. Y. Arkhipova; M. A. Nosenko; N. V. Malyuchenko; R. V. Zvartsev; A. M. Moisenovich; A. S. Zhdanova; T. V. Vasil’eva; E. A. Gorshkova; I. I. Agapov; M. S. Drutskaya; S. A. Nedospasov; M. M. Moisenovich

doi:10.1134/S0006297916110031

Effects of fibroin microcarriers on inflammation and regeneration of deep skin wounds in mice

Authors: Arkhipova A.Y.¹, Nosenko M.A.¹^,2, Malyuchenko N.V.¹, Zvartsev R.V.², Moisenovich A.M.², Zhdanova A.S.¹^,2, Vasil’eva T.V.¹, Gorshkova E.A.¹^,2, Agapov I.I.³, Drutskaya M.S.¹^,2, Nedospasov S.A.¹^,2^,4, Moisenovich M.M.¹
Affiliations:
1. Biological Faculty
2. Engelhardt Institute of Molecular Biology
3. Shumakov Research Institute of Transplantation and Artificial Organs
4. Belozersky Institute of Physico-Chemical Biology
Issue: Vol 81, No 11 (2016)
Pages: 1251-1260
Section: Molecular and Cellular Mechanisms of Inflammation (Special Issue) Guest Editors S. A. Nedospasov and D. V. Kuprash
URL: https://journals.rcsi.science/0006-2979/article/view/151076
DOI: https://doi.org/10.1134/S0006297916110031
ID: 151076

Cite item

Full Text

Open Access
Restricted Access

Access granted
Restricted Access

Subscription Access

Abstract
About the authors
References
Statistics

Abstract

The process of tissue regeneration following damage takes place with direct participation of the immune system. The use of biomaterials as scaffolds to facilitate healing of skin wounds is a new and interesting area of regenerative medicine and biomedical research. In many ways, the regenerative potential of biological material is related to its ability to modulate the inflammatory response. At the same time, all foreign materials, once implanted into a living tissue, to varying degree cause an immune reaction. The modern approach to the development of bioengineered structures for applications in regenerative medicine should be directed toward using the properties of the inflammatory response that improve healing, but do not lead to negative chronic manifestations. In this work, we studied the effect of microcarriers comprised of either fibroin or fibroin supplemented with gelatin on the dynamics of the healing, as well as inflammation, during regeneration of deep skin wounds in mice. We found that subcutaneous administration of microcarriers to the wound area resulted in uniform contraction of the wounds in mice in our experimental model, and microcarrier particles induced the infiltration of immune cells. This was associated with increased expression of proinflammatory cytokines TNF, IL-6, IL-1β, and chemokines CXCL1 and CXCL2, which contributed to full functional recovery of the injured area and the absence of fibrosis as compared to the control group.

Keywords

proinflammatory cytokines, microparticles, fibroin, IL-6, TNF

Username
Password
Remember me

Forgot password?	Register

Username
Password
Remember me

Forgot password?	Register

Effects of fibroin microcarriers on inflammation and regeneration of deep skin wounds in mice

Full Text

Abstract

Keywords

About the authors

This website uses cookies