Procedure for purification of recombinant preMsk1p from E. coli determines its properties as a factor of tRNA import into yeast mitochondria
- Authors: Smirnova E.V.1,2,3, Chicherin I.V.1,2, Baleva M.V.1,2, Entelis N.S.2, Tarassov I.A.2, Kamenski P.A.1
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Affiliations:
- Faculty of Biology
- UMR No. 7156 GMGM
- Institute of Plant Molecular Biology
- Issue: Vol 81, No 10 (2016)
- Pages: 1081-1088
- Section: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151028
- DOI: https://doi.org/10.1134/S0006297916100060
- ID: 151028
Cite item
Abstract
Mitochondrial genomes of many eukaryotic organisms do not code for the full tRNA set necessary for organellar translation. Missing tRNA species are imported from the cytosol. In particular, one out of two cytosolic lysine tRNAs of the yeast Saccharomyces cerevisiae is partially internalized by mitochondria. The key protein factor of this process is the precursor of mitochondrial lysyl-tRNA synthetase, preMsk1p. In this work, we show that recombinant preMsk1p purified from E. coli in native conditions, when used in an in vitro tRNA import system, demonstrates some properties different from those shown by the renatured protein purified from E. coli in the denatured state. We also discuss the possible mechanistic reasons for this phenomenon.
About the authors
E. V. Smirnova
Faculty of Biology; UMR No. 7156 GMGM; Institute of Plant Molecular Biology
Email: kamenski_pa@mail.bio.msu.ru
Russian Federation, Moscow, 119991; 21 rue Rene Descartes, Strasbourg, 67084; 4 rue General Zimmer, Strasbourg, 67084
I. V. Chicherin
Faculty of Biology; UMR No. 7156 GMGM
Email: kamenski_pa@mail.bio.msu.ru
Russian Federation, Moscow, 119991; 21 rue Rene Descartes, Strasbourg, 67084
M. V. Baleva
Faculty of Biology; UMR No. 7156 GMGM
Email: kamenski_pa@mail.bio.msu.ru
Russian Federation, Moscow, 119991; 21 rue Rene Descartes, Strasbourg, 67084
N. S. Entelis
UMR No. 7156 GMGM
Email: kamenski_pa@mail.bio.msu.ru
France, 21 rue Rene Descartes, Strasbourg, 67084
I. A. Tarassov
UMR No. 7156 GMGM
Email: kamenski_pa@mail.bio.msu.ru
France, 21 rue Rene Descartes, Strasbourg, 67084
P. A. Kamenski
Faculty of Biology
Author for correspondence.
Email: kamenski_pa@mail.bio.msu.ru
Russian Federation, Moscow, 119991