Procedure for purification of recombinant preMsk1p from E. coli determines its properties as a factor of tRNA import into yeast mitochondria
- 作者: Smirnova E.1,2,3, Chicherin I.1,2, Baleva M.1,2, Entelis N.2, Tarassov I.2, Kamenski P.1
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隶属关系:
- Faculty of Biology
- UMR No. 7156 GMGM
- Institute of Plant Molecular Biology
- 期: 卷 81, 编号 10 (2016)
- 页面: 1081-1088
- 栏目: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151028
- DOI: https://doi.org/10.1134/S0006297916100060
- ID: 151028
如何引用文章
详细
Mitochondrial genomes of many eukaryotic organisms do not code for the full tRNA set necessary for organellar translation. Missing tRNA species are imported from the cytosol. In particular, one out of two cytosolic lysine tRNAs of the yeast Saccharomyces cerevisiae is partially internalized by mitochondria. The key protein factor of this process is the precursor of mitochondrial lysyl-tRNA synthetase, preMsk1p. In this work, we show that recombinant preMsk1p purified from E. coli in native conditions, when used in an in vitro tRNA import system, demonstrates some properties different from those shown by the renatured protein purified from E. coli in the denatured state. We also discuss the possible mechanistic reasons for this phenomenon.
作者简介
E. Smirnova
Faculty of Biology; UMR No. 7156 GMGM; Institute of Plant Molecular Biology
Email: kamenski_pa@mail.bio.msu.ru
俄罗斯联邦, Moscow, 119991; 21 rue Rene Descartes, Strasbourg, 67084; 4 rue General Zimmer, Strasbourg, 67084
I. Chicherin
Faculty of Biology; UMR No. 7156 GMGM
Email: kamenski_pa@mail.bio.msu.ru
俄罗斯联邦, Moscow, 119991; 21 rue Rene Descartes, Strasbourg, 67084
M. Baleva
Faculty of Biology; UMR No. 7156 GMGM
Email: kamenski_pa@mail.bio.msu.ru
俄罗斯联邦, Moscow, 119991; 21 rue Rene Descartes, Strasbourg, 67084
N. Entelis
UMR No. 7156 GMGM
Email: kamenski_pa@mail.bio.msu.ru
法国, 21 rue Rene Descartes, Strasbourg, 67084
I. Tarassov
UMR No. 7156 GMGM
Email: kamenski_pa@mail.bio.msu.ru
法国, 21 rue Rene Descartes, Strasbourg, 67084
P. Kamenski
Faculty of Biology
编辑信件的主要联系方式.
Email: kamenski_pa@mail.bio.msu.ru
俄罗斯联邦, Moscow, 119991
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