Interaction of telomeric retroelement HeT-A transcripts and their protein product gag in early embryogenesis of Drosophila
- Authors: Olovnikov I.A.1, Morgunova V.V.1, Mironova A.A.1, Kordyukova M.Y.1, Radion E.I.1, Olenkina O.M.1, Akulenko N.V.1, Kalmykova A.I.1
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Affiliations:
- Institute of Molecular Genetics
- Issue: Vol 81, No 9 (2016)
- Pages: 1023-1030
- Section: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151015
- DOI: https://doi.org/10.1134/S000629791609011X
- ID: 151015
Cite item
Abstract
The telomere is a nucleoprotein complex at the ends of linear chromosomes that protects them from fusion and degradation. The telomere consists of telomeric DNA, a protective protein complex and telomeric RNA. Biogenesis of telomeric transcripts in development is still far from being understood. Drosophila telomeres are elongated by a transposition of specialized telomeric retrotransposons that encode proteins. Using transgenic constructs encoding tagged telomeric protein, we found that transcripts of Drosophila telomeric element HeT-A bind Gag-HeT-A protein encoded by these transcripts. Maternal HeT-A transcripts and Gag-HeT-A form ribonucleoprotein granules around centrosomes, centers of microtubule organization, during blastoderm formation, upon disruption of telomere silencing during oogenesis. The specific localization of HeT-A RNA is dependent on microtubules since disruption of microtubules caused delocalization of HeT-A transcripts. This transgenic system is a valuable model for the study of telomeric RNA biogenesis.
Keywords
About the authors
I. A. Olovnikov
Institute of Molecular Genetics
Email: allakalm@img.ras.ru
Russian Federation, Moscow, 123182
V. V. Morgunova
Institute of Molecular Genetics
Email: allakalm@img.ras.ru
Russian Federation, Moscow, 123182
A. A. Mironova
Institute of Molecular Genetics
Email: allakalm@img.ras.ru
Russian Federation, Moscow, 123182
M. Y. Kordyukova
Institute of Molecular Genetics
Email: allakalm@img.ras.ru
Russian Federation, Moscow, 123182
E. I. Radion
Institute of Molecular Genetics
Email: allakalm@img.ras.ru
Russian Federation, Moscow, 123182
O. M. Olenkina
Institute of Molecular Genetics
Email: allakalm@img.ras.ru
Russian Federation, Moscow, 123182
N. V. Akulenko
Institute of Molecular Genetics
Email: allakalm@img.ras.ru
Russian Federation, Moscow, 123182
A. I. Kalmykova
Institute of Molecular Genetics
Author for correspondence.
Email: allakalm@img.ras.ru
Russian Federation, Moscow, 123182