Interaction of telomeric retroelement HeT-A transcripts and their protein product gag in early embryogenesis of Drosophila
- Авторы: Olovnikov I.1, Morgunova V.1, Mironova A.1, Kordyukova M.1, Radion E.1, Olenkina O.1, Akulenko N.1, Kalmykova A.1
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Учреждения:
- Institute of Molecular Genetics
- Выпуск: Том 81, № 9 (2016)
- Страницы: 1023-1030
- Раздел: Article
- URL: https://journals.rcsi.science/0006-2979/article/view/151015
- DOI: https://doi.org/10.1134/S000629791609011X
- ID: 151015
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Аннотация
The telomere is a nucleoprotein complex at the ends of linear chromosomes that protects them from fusion and degradation. The telomere consists of telomeric DNA, a protective protein complex and telomeric RNA. Biogenesis of telomeric transcripts in development is still far from being understood. Drosophila telomeres are elongated by a transposition of specialized telomeric retrotransposons that encode proteins. Using transgenic constructs encoding tagged telomeric protein, we found that transcripts of Drosophila telomeric element HeT-A bind Gag-HeT-A protein encoded by these transcripts. Maternal HeT-A transcripts and Gag-HeT-A form ribonucleoprotein granules around centrosomes, centers of microtubule organization, during blastoderm formation, upon disruption of telomere silencing during oogenesis. The specific localization of HeT-A RNA is dependent on microtubules since disruption of microtubules caused delocalization of HeT-A transcripts. This transgenic system is a valuable model for the study of telomeric RNA biogenesis.
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Об авторах
I. Olovnikov
Institute of Molecular Genetics
Email: allakalm@img.ras.ru
Россия, Moscow, 123182
V. Morgunova
Institute of Molecular Genetics
Email: allakalm@img.ras.ru
Россия, Moscow, 123182
A. Mironova
Institute of Molecular Genetics
Email: allakalm@img.ras.ru
Россия, Moscow, 123182
M. Kordyukova
Institute of Molecular Genetics
Email: allakalm@img.ras.ru
Россия, Moscow, 123182
E. Radion
Institute of Molecular Genetics
Email: allakalm@img.ras.ru
Россия, Moscow, 123182
O. Olenkina
Institute of Molecular Genetics
Email: allakalm@img.ras.ru
Россия, Moscow, 123182
N. Akulenko
Institute of Molecular Genetics
Email: allakalm@img.ras.ru
Россия, Moscow, 123182
A. Kalmykova
Institute of Molecular Genetics
Автор, ответственный за переписку.
Email: allakalm@img.ras.ru
Россия, Moscow, 123182