Enviar artigo por via de e-mail Compatibility of cells of the nervous system with structured biodegradable chitosan-based hydrogel matrices
- Autores: Timashev P.S.1, Bardakova K.N.1, Minaev N.V.1, Demina T.S.2, Mishchenko T.A.3, Mitroshina E.V.3, Akovantseva A.A.1, Koroleva A.V.1, Asyutin D.S.4, Pimenova L.F.4, Konovalov N.A.4, Akopova T.A.2, Solov’eva A.B.5, Mukhina I.V.3, Vedunova M.V.6, Chichkov B.N.1, Bagratashvili V.N.1
-
Afiliações:
- Institute of Laser and Information Technologies, Department of Advanced Laser Technology
- Enikolopov Institute of Synthetic Polymeric Materials
- Nizhny Novgorod State Medical Academy
- Burdenko Research Institute of Neurosurgery
- Semenov Institute of Chemical Physics
- Institute of Biology and Biomedicine
- Edição: Volume 52, Nº 5 (2016)
- Páginas: 508-514
- Seção: Article
- URL: https://journals.rcsi.science/0003-6838/article/view/152009
- DOI: https://doi.org/10.1134/S0003683816050161
- ID: 152009
Citar
Acesso aberto
Acesso está concedido
Somente assinantes
Resumo
Hydrogel matrices for cell cultivation have been generated by two-photon laser polymerization of unsaturated chitosan derivatives and methacrylated hyaluronic acid. The adhesive and toxic properties of the matrices have been assessed, and the matrices have been shown to have a good compatibility with primary hippocampal cell cultures. The formation of morphologically normal neural networks by cells of the nervous system cultured on the surface of hydrogel matrices has been observed. The metabolic status of dissociated hippocampal cells cultured on the matrices was similar to that of the control cultures, as shown by the results of MTT reductase activity assay. Thus, matrices based on unsaturated polysaccharide derivatives crosslinked by laser irradiation showed good compatibility with differentiated cells of the nervous system and considerable potential for use in neurotransplantation.
Palavras-chave
Sobre autores
P. Timashev
Institute of Laser and Information Technologies, Department of Advanced Laser Technology
Autor responsável pela correspondência
Email: timashev.peter@gmail.com
Rússia, Troitsk, Moscow, 108840
K. Bardakova
Institute of Laser and Information Technologies, Department of Advanced Laser Technology
Email: timashev.peter@gmail.com
Rússia, Troitsk, Moscow, 108840
N. Minaev
Institute of Laser and Information Technologies, Department of Advanced Laser Technology
Email: timashev.peter@gmail.com
Rússia, Troitsk, Moscow, 108840
T. Demina
Enikolopov Institute of Synthetic Polymeric Materials
Email: timashev.peter@gmail.com
Rússia, Moscow, 117393
T. Mishchenko
Nizhny Novgorod State Medical Academy
Email: timashev.peter@gmail.com
Rússia, Nizhny Novgorod, 603005
E. Mitroshina
Nizhny Novgorod State Medical Academy
Email: timashev.peter@gmail.com
Rússia, Nizhny Novgorod, 603005
A. Akovantseva
Institute of Laser and Information Technologies, Department of Advanced Laser Technology
Email: timashev.peter@gmail.com
Rússia, Troitsk, Moscow, 108840
A. Koroleva
Institute of Laser and Information Technologies, Department of Advanced Laser Technology
Email: timashev.peter@gmail.com
Rússia, Troitsk, Moscow, 108840
D. Asyutin
Burdenko Research Institute of Neurosurgery
Email: timashev.peter@gmail.com
Rússia, Moscow, 125047
L. Pimenova
Burdenko Research Institute of Neurosurgery
Email: timashev.peter@gmail.com
Rússia, Moscow, 125047
N. Konovalov
Burdenko Research Institute of Neurosurgery
Email: timashev.peter@gmail.com
Rússia, Moscow, 125047
T. Akopova
Enikolopov Institute of Synthetic Polymeric Materials
Email: timashev.peter@gmail.com
Rússia, Moscow, 117393
A. Solov’eva
Semenov Institute of Chemical Physics
Email: timashev.peter@gmail.com
Rússia, Moscow, 119991
I. Mukhina
Nizhny Novgorod State Medical Academy
Email: timashev.peter@gmail.com
Rússia, Nizhny Novgorod, 603005
M. Vedunova
Institute of Biology and Biomedicine
Email: timashev.peter@gmail.com
Rússia, Nizhny Novgorod, 603950
B. Chichkov
Institute of Laser and Information Technologies, Department of Advanced Laser Technology
Email: timashev.peter@gmail.com
Rússia, Troitsk, Moscow, 108840
V. Bagratashvili
Institute of Laser and Information Technologies, Department of Advanced Laser Technology
Email: timashev.peter@gmail.com
Rússia, Troitsk, Moscow, 108840
Arquivos suplementares
