Мақаланы E-mail арқылы жіберу Compatibility of cells of the nervous system with structured biodegradable chitosan-based hydrogel matrices
- Авторлар: Timashev P.S.1, Bardakova K.N.1, Minaev N.V.1, Demina T.S.2, Mishchenko T.A.3, Mitroshina E.V.3, Akovantseva A.A.1, Koroleva A.V.1, Asyutin D.S.4, Pimenova L.F.4, Konovalov N.A.4, Akopova T.A.2, Solov’eva A.B.5, Mukhina I.V.3, Vedunova M.V.6, Chichkov B.N.1, Bagratashvili V.N.1
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Мекемелер:
- Institute of Laser and Information Technologies, Department of Advanced Laser Technology
- Enikolopov Institute of Synthetic Polymeric Materials
- Nizhny Novgorod State Medical Academy
- Burdenko Research Institute of Neurosurgery
- Semenov Institute of Chemical Physics
- Institute of Biology and Biomedicine
- Шығарылым: Том 52, № 5 (2016)
- Беттер: 508-514
- Бөлім: Article
- URL: https://journals.rcsi.science/0003-6838/article/view/152009
- DOI: https://doi.org/10.1134/S0003683816050161
- ID: 152009
Дәйексөз келтіру
Ашық рұқсат
Рұқсат берілді
Тек жазылушылар үшін
Аннотация
Hydrogel matrices for cell cultivation have been generated by two-photon laser polymerization of unsaturated chitosan derivatives and methacrylated hyaluronic acid. The adhesive and toxic properties of the matrices have been assessed, and the matrices have been shown to have a good compatibility with primary hippocampal cell cultures. The formation of morphologically normal neural networks by cells of the nervous system cultured on the surface of hydrogel matrices has been observed. The metabolic status of dissociated hippocampal cells cultured on the matrices was similar to that of the control cultures, as shown by the results of MTT reductase activity assay. Thus, matrices based on unsaturated polysaccharide derivatives crosslinked by laser irradiation showed good compatibility with differentiated cells of the nervous system and considerable potential for use in neurotransplantation.
Негізгі сөздер
Авторлар туралы
P. Timashev
Institute of Laser and Information Technologies, Department of Advanced Laser Technology
Хат алмасуға жауапты Автор.
Email: timashev.peter@gmail.com
Ресей, Troitsk, Moscow, 108840
K. Bardakova
Institute of Laser and Information Technologies, Department of Advanced Laser Technology
Email: timashev.peter@gmail.com
Ресей, Troitsk, Moscow, 108840
N. Minaev
Institute of Laser and Information Technologies, Department of Advanced Laser Technology
Email: timashev.peter@gmail.com
Ресей, Troitsk, Moscow, 108840
T. Demina
Enikolopov Institute of Synthetic Polymeric Materials
Email: timashev.peter@gmail.com
Ресей, Moscow, 117393
T. Mishchenko
Nizhny Novgorod State Medical Academy
Email: timashev.peter@gmail.com
Ресей, Nizhny Novgorod, 603005
E. Mitroshina
Nizhny Novgorod State Medical Academy
Email: timashev.peter@gmail.com
Ресей, Nizhny Novgorod, 603005
A. Akovantseva
Institute of Laser and Information Technologies, Department of Advanced Laser Technology
Email: timashev.peter@gmail.com
Ресей, Troitsk, Moscow, 108840
A. Koroleva
Institute of Laser and Information Technologies, Department of Advanced Laser Technology
Email: timashev.peter@gmail.com
Ресей, Troitsk, Moscow, 108840
D. Asyutin
Burdenko Research Institute of Neurosurgery
Email: timashev.peter@gmail.com
Ресей, Moscow, 125047
L. Pimenova
Burdenko Research Institute of Neurosurgery
Email: timashev.peter@gmail.com
Ресей, Moscow, 125047
N. Konovalov
Burdenko Research Institute of Neurosurgery
Email: timashev.peter@gmail.com
Ресей, Moscow, 125047
T. Akopova
Enikolopov Institute of Synthetic Polymeric Materials
Email: timashev.peter@gmail.com
Ресей, Moscow, 117393
A. Solov’eva
Semenov Institute of Chemical Physics
Email: timashev.peter@gmail.com
Ресей, Moscow, 119991
I. Mukhina
Nizhny Novgorod State Medical Academy
Email: timashev.peter@gmail.com
Ресей, Nizhny Novgorod, 603005
M. Vedunova
Institute of Biology and Biomedicine
Email: timashev.peter@gmail.com
Ресей, Nizhny Novgorod, 603950
B. Chichkov
Institute of Laser and Information Technologies, Department of Advanced Laser Technology
Email: timashev.peter@gmail.com
Ресей, Troitsk, Moscow, 108840
V. Bagratashvili
Institute of Laser and Information Technologies, Department of Advanced Laser Technology
Email: timashev.peter@gmail.com
Ресей, Troitsk, Moscow, 108840
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