Compatibility of cells of the nervous system with structured biodegradable chitosan-based hydrogel matrices

P. S. Timashev; K. N. Bardakova; N. V. Minaev; T. S. Demina; T. A. Mishchenko; E. V. Mitroshina; A. A. Akovantseva; A. V. Koroleva; D. S. Asyutin; L. F. Pimenova; N. A. Konovalov; T. A. Akopova; A. B. Solov’eva; I. V. Mukhina; M. V. Vedunova; B. N. Chichkov; V. N. Bagratashvili

doi:10.1134/S0003683816050161

Compatibility of cells of the nervous system with structured biodegradable chitosan-based hydrogel matrices

Authors: Timashev P.S.¹, Bardakova K.N.¹, Minaev N.V.¹, Demina T.S.², Mishchenko T.A.³, Mitroshina E.V.³, Akovantseva A.A.¹, Koroleva A.V.¹, Asyutin D.S.⁴, Pimenova L.F.⁴, Konovalov N.A.⁴, Akopova T.A.², Solov’eva A.B.⁵, Mukhina I.V.³, Vedunova M.V.⁶, Chichkov B.N.¹, Bagratashvili V.N.¹
Affiliations:
1. Institute of Laser and Information Technologies, Department of Advanced Laser Technology
2. Enikolopov Institute of Synthetic Polymeric Materials
3. Nizhny Novgorod State Medical Academy
4. Burdenko Research Institute of Neurosurgery
5. Semenov Institute of Chemical Physics
6. Institute of Biology and Biomedicine
Issue: Vol 52, No 5 (2016)
Pages: 508-514
Section: Article
URL: https://journals.rcsi.science/0003-6838/article/view/152009
DOI: https://doi.org/10.1134/S0003683816050161
ID: 152009

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Abstract

Hydrogel matrices for cell cultivation have been generated by two-photon laser polymerization of unsaturated chitosan derivatives and methacrylated hyaluronic acid. The adhesive and toxic properties of the matrices have been assessed, and the matrices have been shown to have a good compatibility with primary hippocampal cell cultures. The formation of morphologically normal neural networks by cells of the nervous system cultured on the surface of hydrogel matrices has been observed. The metabolic status of dissociated hippocampal cells cultured on the matrices was similar to that of the control cultures, as shown by the results of MTT reductase activity assay. Thus, matrices based on unsaturated polysaccharide derivatives crosslinked by laser irradiation showed good compatibility with differentiated cells of the nervous system and considerable potential for use in neurotransplantation.

Keywords

structured hydrogels, chitosan, two-photon polymerization, neural networks, regenerative therapy, scaffolds

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Compatibility of cells of the nervous system with structured biodegradable chitosan-based hydrogel matrices

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Abstract

Keywords

About the authors

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