Physicochemical Characteristics of a Variant of Chaperon GroEL Apical Domain Designed to Enhance the Expression and Stability of Target Proteins
- Authors: Kurov K.A.1, Savvin O.I.1, Yurkova M.S.2,1, Zenin V.A.2,1, Nagibina G.S.3, Melnik B.S.3, Fedorov A.N.2,1
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Affiliations:
- Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences
- People’s Friendship University of Russia
- Institute of Protein Research, Russian Academy of Sciences
- Issue: Vol 55, No 8 (2019)
- Pages: 765-770
- Section: Producers, Biology, Selection, and Gene Engineering
- URL: https://journals.rcsi.science/0003-6838/article/view/153101
- DOI: https://doi.org/10.1134/S0003683819080088
- ID: 153101
Cite item
Abstract
This work describes the properties of a new protein, a modification of GroEL apical domain designed to be a leader in fusion systems. This polypeptide leader demonstrates a high level of expression in a bacterial system; it is soluble and retains its solubility during standard biochemical manipulations. The secondary structure of the protein and its thermostability, as well as the protein solubility, were studied in a wide temperature range. To simplify the subsequent purification of the target protein, the possibility of its chemical cleavage from the fused protein by methionine residues with cyanogen bromide is provided.
About the authors
K. A. Kurov
Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences
Email: a.fedorov@fbras.ru
Russian Federation, Moscow, 119071
O. I. Savvin
Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences
Email: a.fedorov@fbras.ru
Russian Federation, Moscow, 119071
M. S. Yurkova
People’s Friendship University of Russia; Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences
Email: a.fedorov@fbras.ru
Russian Federation, Moscow, 117198; Moscow, 119071
V. A. Zenin
People’s Friendship University of Russia; Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences
Email: a.fedorov@fbras.ru
Russian Federation, Moscow, 117198; Moscow, 119071
G. S. Nagibina
Institute of Protein Research, Russian Academy of Sciences
Email: a.fedorov@fbras.ru
Russian Federation, Pushchino, Moscow oblast, 142290
B. S. Melnik
Institute of Protein Research, Russian Academy of Sciences
Email: a.fedorov@fbras.ru
Russian Federation, Pushchino, Moscow oblast, 142290
A. N. Fedorov
People’s Friendship University of Russia; Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences
Author for correspondence.
Email: a.fedorov@fbras.ru
Russian Federation, Moscow, 117198; Moscow, 119071
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