Physicochemical Characteristics of a Variant of Chaperon GroEL Apical Domain Designed to Enhance the Expression and Stability of Target Proteins


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Abstract

This work describes the properties of a new protein, a modification of GroEL apical domain designed to be a leader in fusion systems. This polypeptide leader demonstrates a high level of expression in a bacterial system; it is soluble and retains its solubility during standard biochemical manipulations. The secondary structure of the protein and its thermostability, as well as the protein solubility, were studied in a wide temperature range. To simplify the subsequent purification of the target protein, the possibility of its chemical cleavage from the fused protein by methionine residues with cyanogen bromide is provided.

About the authors

K. A. Kurov

Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences

Email: a.fedorov@fbras.ru
Russian Federation, Moscow, 119071

O. I. Savvin

Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences

Email: a.fedorov@fbras.ru
Russian Federation, Moscow, 119071

M. S. Yurkova

People’s Friendship University of Russia; Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences

Email: a.fedorov@fbras.ru
Russian Federation, Moscow, 117198; Moscow, 119071

V. A. Zenin

People’s Friendship University of Russia; Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences

Email: a.fedorov@fbras.ru
Russian Federation, Moscow, 117198; Moscow, 119071

G. S. Nagibina

Institute of Protein Research, Russian Academy of Sciences

Email: a.fedorov@fbras.ru
Russian Federation, Pushchino, Moscow oblast, 142290

B. S. Melnik

Institute of Protein Research, Russian Academy of Sciences

Email: a.fedorov@fbras.ru
Russian Federation, Pushchino, Moscow oblast, 142290

A. N. Fedorov

People’s Friendship University of Russia; Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences

Author for correspondence.
Email: a.fedorov@fbras.ru
Russian Federation, Moscow, 117198; Moscow, 119071

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