Effect of the Method of Nanocomplex Synthesis on the Proteolytic Activity of Chymotrypsin Immobilized on Silver Nanoparticles


Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription Access

Abstract

Two methods for the synthesis of hybrid organic-inorganic nanocomplexes comprising chymotrypsin and silver nanoparticles have been developed based on the chemical reduction of silver nitrate by sodium borohydride. In the first method, the enzyme is added to the reaction mixture simultaneously with other reagents. In the second method, it is added to the reaction mixture at certain intervals after the start of the reduction reaction. Chymotrypsin immobilized on nanocomplexes stabilizes silver nanoparticles. As compared to the native enzyme, the immobilized enzyme prepared by the both methods exhibits an increased proteolytic activity in acidic and strongly alkaline media. At the same time, chymotrypsin immobilized on silver nanoparticles by the second method exhibits the highest level of proteolytic activity.

About the authors

A. V. Plyushchenko

Institute of Macromolecular Compounds, Russian Academy of Sciences; Peter the Great St. Petersburg Polytechnic University

Email: chrom79@yandex.ru
Russian Federation, St. Petersburg, 199004; St. Petersburg, 195251

L. N. Borovikova

Institute of Macromolecular Compounds, Russian Academy of Sciences

Email: chrom79@yandex.ru
Russian Federation, St. Petersburg, 199004

O. A. Pisarev

Institute of Macromolecular Compounds, Russian Academy of Sciences; Peter the Great St. Petersburg Polytechnic University

Author for correspondence.
Email: chrom79@yandex.ru
Russian Federation, St. Petersburg, 199004; St. Petersburg, 195251

Supplementary files

Supplementary Files
Action
1. JATS XML

Copyright (c) 2019 Pleiades Publishing, Inc.