1,3-Diaminopropane and Spermidine Upregulate Lovastatin Production and Expression of Lovastatin Biosynthetic Genes in Aspergillus terreus via LaeA Regulation
- Authors: Zhgun A.A.1, Nuraeva G.K.1, Dumina M.V.1, Voinova T.M.2, Dzhavakhiya V.V.1, Eldarov M.A.1
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Affiliations:
- Institute of Bioengineering, Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences
- All-Russia Research Institute of Phytopathology
- Issue: Vol 55, No 3 (2019)
- Pages: 243-254
- Section: Article
- URL: https://journals.rcsi.science/0003-6838/article/view/152870
- DOI: https://doi.org/10.1134/S0003683819020170
- ID: 152870
Cite item
Abstract
The filamentous fungus Aspergillus terreus is the main industrial producer of lovastatin, a cholesterol-lowering drug that is also used as a semiproduct for simvastatin production. The exogenous addition of such polyamines as 1,3-diaminopropane or spermidine during the fermentation of wild-type A. terreus ATCC 20542 and overproducing A. terreus 43-16 strains results in a 20−45% increase in lovastatin production. In the case of strain 43-16, the maximum production level (10 g/L) was observed three days earlier than in the control variant. During this fermentation period, the expression level of genes belonging to the lovastatin biosynthetic cluster and the laeA gene (a global regulator of a fungal secondary metabolism) are increased, but the expression patterns for genes of the polyamine metabolic pathway were similar for both strains.
About the authors
A. A. Zhgun
Institute of Bioengineering, Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences
Author for correspondence.
Email: Zzhgun@mail.ru
Russian Federation, Moscow, 117312
G. K. Nuraeva
Institute of Bioengineering, Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences
Email: Zzhgun@mail.ru
Russian Federation, Moscow, 117312
M. V. Dumina
Institute of Bioengineering, Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences
Email: Zzhgun@mail.ru
Russian Federation, Moscow, 117312
T. M. Voinova
All-Russia Research Institute of Phytopathology
Email: Zzhgun@mail.ru
Russian Federation, Bolshie Vyazemy, Moscow oblast, 143050
V. V. Dzhavakhiya
Institute of Bioengineering, Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences
Email: Zzhgun@mail.ru
Russian Federation, Moscow, 117312
M. A. Eldarov
Institute of Bioengineering, Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences
Email: Zzhgun@mail.ru
Russian Federation, Moscow, 117312
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