Generation and Characterization of the Monoclonal Antibody Panel Specific to the NS1 Protein of the Influenza A Virus


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The paper reports on the production of a panel of seven new monoclonal antibodies (MAbs) capable of specifically binding to the NS11–124 fragment of the influenza virus A/Puerto Rico/8/1934 (H1N1) NS1 protein in indirect enzyme-linked immunosorbent assay (ELISA), as well as to the full-size native NS1 protein in cells infected with different subtypes of the influenza A virus (A(H1N1), A(H1N1)pdm09, A(H2N2), A(H3N2), and A(H9N2)). A sensitive variant of the microcultural ELISA based on the use of the generated MAbs is proposed; it may be utilized to detect NS1 protein in infected cells and to study its antigenic variability. The results of the performed microcultural ELISA demonstrate type- and subtype-specific antigenic variability of the influenza virus NS1 protein.

作者简介

V. Krivitskaya

Research Institute of Influenza, Ministry of Health Care of the Russian Federation (NII Grippa)

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Email: vera.kriv@influenza.spb.ru
俄罗斯联邦, St. Petersburg, 197376

N. Konovalova

Research Institute of Influenza, Ministry of Health Care of the Russian Federation (NII Grippa)

Email: vera.kriv@influenza.spb.ru
俄罗斯联邦, St. Petersburg, 197376

E. Petrova

Research Institute of Influenza, Ministry of Health Care of the Russian Federation (NII Grippa)

Email: vera.kriv@influenza.spb.ru
俄罗斯联邦, St. Petersburg, 197376

S. Petrov

Research Institute of Influenza, Ministry of Health Care of the Russian Federation (NII Grippa)

Email: vera.kriv@influenza.spb.ru
俄罗斯联邦, St. Petersburg, 197376

A. Shaldzhyan

Research Institute of Influenza, Ministry of Health Care of the Russian Federation (NII Grippa)

Email: vera.kriv@influenza.spb.ru
俄罗斯联邦, St. Petersburg, 197376

E. Romanovskaya-Roman’ko

Research Institute of Influenza, Ministry of Health Care of the Russian Federation (NII Grippa)

Email: vera.kriv@influenza.spb.ru
俄罗斯联邦, St. Petersburg, 197376

R. Kadyrova

Research Institute of Influenza, Ministry of Health Care of the Russian Federation (NII Grippa)

Email: vera.kriv@influenza.spb.ru
俄罗斯联邦, St. Petersburg, 197376

M. Sergeeva

Research Institute of Influenza, Ministry of Health Care of the Russian Federation (NII Grippa)

Email: vera.kriv@influenza.spb.ru
俄罗斯联邦, St. Petersburg, 197376

T. Tsareva

Research Institute of Influenza, Ministry of Health Care of the Russian Federation (NII Grippa)

Email: vera.kriv@influenza.spb.ru
俄罗斯联邦, St. Petersburg, 197376

E. Sorokin

Research Institute of Influenza, Ministry of Health Care of the Russian Federation (NII Grippa)

Email: vera.kriv@influenza.spb.ru
俄罗斯联邦, St. Petersburg, 197376

P. Petrova

Research Institute of Influenza, Ministry of Health Care of the Russian Federation (NII Grippa)

Email: vera.kriv@influenza.spb.ru
俄罗斯联邦, St. Petersburg, 197376

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