Generation and Characterization of the Monoclonal Antibody Panel Specific to the NS1 Protein of the Influenza A Virus
- 作者: Krivitskaya V.Z.1, Konovalova N.I.1, Petrova E.R.1, Petrov S.V.1, Shaldzhyan A.A.1, Romanovskaya-Roman’ko E.A.1, Kadyrova R.A.1, Sergeeva M.V.1, Tsareva T.R.1, Sorokin E.V.1, Petrova P.A.1
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隶属关系:
- Research Institute of Influenza, Ministry of Health Care of the Russian Federation (NII Grippa)
- 期: 卷 54, 编号 7 (2018)
- 页面: 756-765
- 栏目: Metrology, Standardization, and Control
- URL: https://journals.rcsi.science/0003-6838/article/view/152719
- DOI: https://doi.org/10.1134/S0003683818070049
- ID: 152719
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详细
The paper reports on the production of a panel of seven new monoclonal antibodies (MAbs) capable of specifically binding to the NS11–124 fragment of the influenza virus A/Puerto Rico/8/1934 (H1N1) NS1 protein in indirect enzyme-linked immunosorbent assay (ELISA), as well as to the full-size native NS1 protein in cells infected with different subtypes of the influenza A virus (A(H1N1), A(H1N1)pdm09, A(H2N2), A(H3N2), and A(H9N2)). A sensitive variant of the microcultural ELISA based on the use of the generated MAbs is proposed; it may be utilized to detect NS1 protein in infected cells and to study its antigenic variability. The results of the performed microcultural ELISA demonstrate type- and subtype-specific antigenic variability of the influenza virus NS1 protein.
作者简介
V. Krivitskaya
Research Institute of Influenza, Ministry of Health Care of the Russian Federation (NII Grippa)
编辑信件的主要联系方式.
Email: vera.kriv@influenza.spb.ru
俄罗斯联邦, St. Petersburg, 197376
N. Konovalova
Research Institute of Influenza, Ministry of Health Care of the Russian Federation (NII Grippa)
Email: vera.kriv@influenza.spb.ru
俄罗斯联邦, St. Petersburg, 197376
E. Petrova
Research Institute of Influenza, Ministry of Health Care of the Russian Federation (NII Grippa)
Email: vera.kriv@influenza.spb.ru
俄罗斯联邦, St. Petersburg, 197376
S. Petrov
Research Institute of Influenza, Ministry of Health Care of the Russian Federation (NII Grippa)
Email: vera.kriv@influenza.spb.ru
俄罗斯联邦, St. Petersburg, 197376
A. Shaldzhyan
Research Institute of Influenza, Ministry of Health Care of the Russian Federation (NII Grippa)
Email: vera.kriv@influenza.spb.ru
俄罗斯联邦, St. Petersburg, 197376
E. Romanovskaya-Roman’ko
Research Institute of Influenza, Ministry of Health Care of the Russian Federation (NII Grippa)
Email: vera.kriv@influenza.spb.ru
俄罗斯联邦, St. Petersburg, 197376
R. Kadyrova
Research Institute of Influenza, Ministry of Health Care of the Russian Federation (NII Grippa)
Email: vera.kriv@influenza.spb.ru
俄罗斯联邦, St. Petersburg, 197376
M. Sergeeva
Research Institute of Influenza, Ministry of Health Care of the Russian Federation (NII Grippa)
Email: vera.kriv@influenza.spb.ru
俄罗斯联邦, St. Petersburg, 197376
T. Tsareva
Research Institute of Influenza, Ministry of Health Care of the Russian Federation (NII Grippa)
Email: vera.kriv@influenza.spb.ru
俄罗斯联邦, St. Petersburg, 197376
E. Sorokin
Research Institute of Influenza, Ministry of Health Care of the Russian Federation (NII Grippa)
Email: vera.kriv@influenza.spb.ru
俄罗斯联邦, St. Petersburg, 197376
P. Petrova
Research Institute of Influenza, Ministry of Health Care of the Russian Federation (NII Grippa)
Email: vera.kriv@influenza.spb.ru
俄罗斯联邦, St. Petersburg, 197376
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