Yeast Debaryomyces hansenii within ORMOSIL Shells as a Heterogeneous Biocatalyst


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The relevant sol-gel encapsulation methods to design ORMOSIL (organically modified silica) protective shells around Debaryomyces hansenii yeast cells have been studied. Encapsulation is based on spontaneous “cell-in-shell” 3D structures. The physiological activity of the sol-gel encapsulated cells was determined by measurement of the oxidative activity of a BOD biosensor containing ORMOSIL-encapsulated cells. It was shown that the shells around the microbial cells did not prevent substrate diffusion and had excellent protection from extreme environmental factors (heavy metal ions, high pH, and UV radiation). In addition, it was found that the BOD biosensor surpasses some other known biosensors in their basic characteristics, including sensitivity, stability, and reproducibility. These results are important for the development of innovative methods of cell encapsulation and the creation of biocatalysts used in biotechnology and ecology.

作者简介

O. Ponamoreva

Tula State University

编辑信件的主要联系方式.
Email: olgaponamoreva@mail.ru
俄罗斯联邦, Tula, 300012

E. Afonina

Tula State University

Email: olgaponamoreva@mail.ru
俄罗斯联邦, Tula, 300012

O. Kamanina

Tula State University

Email: olgaponamoreva@mail.ru
俄罗斯联邦, Tula, 300012

D. Lavrova

Tula State University

Email: olgaponamoreva@mail.ru
俄罗斯联邦, Tula, 300012

V. Arlyapov

Tula State University

Email: olgaponamoreva@mail.ru
俄罗斯联邦, Tula, 300012

V. Alferov

Tula State University

Email: olgaponamoreva@mail.ru
俄罗斯联邦, Tula, 300012

A. Boronin

Skryabin Institute for Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences

Email: olgaponamoreva@mail.ru
俄罗斯联邦, Pushchino, Moskovskaya oblast, 142290

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