Screening and Taxonomic Characterization of Xylanase Bacterial Producers

Abstract

A stepwise screening of bacterial xylanase producers isolated from forest soil in Moscow oblast has been performed, and the taxa of the microorganisms have been identified based on an analysis of the 16S RNA gene sequences. An approach to the selection of the most active producers is suggested, and this selection has been carried out, resulting in four producing strains. Interestingly, the species of two of the selected strains (Paenibacillus jamilae and P. brasilensis) are described as producers of xylanolytic enzymes for the first time, and the characteristics of those xylanases have not yet been studied. The new strains can serve as a source of genes for the construction of high-efficient xylanase producers.