Gallbladder microbiota in patients with gallstone disease

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Abstract

The article presents the results of investigating 20 patients with cholelithiasis. The bile and a piece of the gallbladder were taken to analyze microbiota during a scheduled laparoscopic cholecystectomy. The study of microbiota was carried out with the cultural method and real-time PCR. Bifidobacterium spp., Bacteroides spp., Lactobacillus spp., E. coli prevailed in the taxonomic structure of isolated bacteria. Isolated Enterococcus spp. had a lot of genes encoding various factors of pathogenicity.

About the authors

Vladimir I. Simanenkov

North-West State Medical University named after II Mechnikov, Ministry of Health of Russia

Email: visimanenkov@mail.ru
ORCID iD: 0000-0002-1956-0070
SPIN-code: 8073-2401

MD, professor

Russian Federation, St. Petersburg

Alexandr N. Suvorov

Institute of Experimental Medicine

Email: lermolenko@yandex.ru
ORCID iD: 0000-0003-2312-5589
SPIN-code: 8062-5281
Scopus Author ID: 7101829979
ResearcherId: J-6921-2013

MD, professor, Corresponding Member of the Russian Academy of Sciences,

Russian Federation, St. Petersburg

Sergey V. Tikhonov

North-West State Medical University named after II Mechnikov, Ministry of Health of Russia

Email: sergeyvt2702@gmail.com
SPIN-code: 6921-5511

MC, associate professor

Russian Federation, St. Petersburg

Elena I. Ermolenko

Institute of Experimental Medicine

Email: lermolenko@yandex.ru
SPIN-code: 9905-0590
Scopus Author ID: 13004322500

MD, professor

Russian Federation, St. Petersburg

Viktoria D. Dekkanova

North-West State Medical University named after I. I. Mechnikov, Ministry of Health of Russia

Author for correspondence.
Email: dekkanova@mail.ru
ORCID iD: 0000-0003-4949-6361
SPIN-code: 5193-4350

Clinical Resident

Russian Federation, St. Petersburg

Artemiy E. Goncharov

North-West State Medical University named after I. I. Mechnikov, Ministry of Health of Russia; Institute of Experimental Medicine

Email: phage1@yandex.ru
ORCID iD: 0000-0002-5206-6656
SPIN-code: 7909-5446
Scopus Author ID: 14025260100
ResearcherId: H-3434-2013

MD, professor

Russian Federation, St. Petersburg

Pavel V. Tkachev

Institute of Experimental Medicine

Email: phage1@yandex.ru

graduate student

Russian Federation, St. Petersburg

Nataliay V. Bakulina

North-West State Medical University named after I.I. Mechnikov, Ministry of Health of Russia

Email: nv_bakulina@mail.ru
ORCID iD: 0000-0003-4075-4096
Scopus Author ID: 7201739080
ResearcherId: N-7299-2014
http://www.researcherid.com/rid/N-7299-2014

MD, professor

Russian Federation, St. Petersburg

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Supplementary files

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2. Fig. 1. The structure of bacterial taxa identified by the PCR in the tissues of the removed gallbladder in the patients with gallstone disease

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3. Fig. 2. Possible mechanisms of the influence of dysbiotic disorders on the development of gallstone disease

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Copyright (c) 2020 Simanenkov V.I., Suvorov A.N., Tikhonov S.V., Ermolenko E.I., Dekkanova V.D., Goncharov A.E., Tkachev P.V., Bakulina N.V.

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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