The use of E. faecium probiotic and autoprobiotic in patients with type 2 diabetes mellitus
- Authors: Bakulina N.V.1, Tikhonov S.V.2, Ermolenko E.I.3, Kotyleva M.P.3, Lavrenova N.S.3, Topalova Y.G.1, Simanenkov V.I.1, Suvorov A.N.3
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Affiliations:
- North-Western State Medical University named after I.I. Mechnikov
- North-Western State Medical University named after I.I. Mechniko
- Institute of Experimental Medicine
- Issue: Vol 14, No 1 (2022)
- Pages: 77-88
- Section: Original research
- URL: https://journals.rcsi.science/vszgmu/article/view/104795
- DOI: https://doi.org/10.17816/mechnikov104795
- ID: 104795
Cite item
Abstract
BACKGROUND: Bacteria in the gastrointestinal tract regulate eating behavior and metabolic processes. A number of probiotic strains have a positive effect on glucose and insulin metabolism. A series of studies have demonstrated the effectiveness of autoprobiotic therapy in the treatment of patients with gastrointestinal diseases. The efficacy and safety of an indigenous strain of Enterococcus faecium in overweight patients with type 2 diabetes has not been studied in the past.
AIM: To evaluate the effectiveness of products containing the probiotic strain Enterococcus faecium L3 or the indigenous strain Enterococcus faecium in patients with diabetes mellitus (DM) type 2.
MATERIALS AND METHODS: Patients with diabetes mellitus type 2 were randomized into two groups: group of probiotic therapy with an industrial strain of Enterococcus faecium L3 (11 patients); group of autoprobiotic therapy based on an indigenous strain of Enterococcus faecium (9 patients). Therapy was for 14 days. Before and 10-14 days after the end of therapy, anthropometric parameters were assessed; psychometric testing was carried out; biochemical parameters of blood serum were studied. The intestinal microbiota was studied by real-time polymerase chain reaction.
RESULTS: Probiotic therapy based on Enterococcus faecium L3 and autoprobiotic therapy based on indigenous Enterococcus faecium had no significant effect on glucose metabolism in patients with type 2 DM. In the Enterococcus faecium L3 group and the group of indigenous Enterococcus faecium there was a significant decrease in gastroenterological complaints on scales of the GSRS questionnaire. Patients in both groups showed a statistically significant decrease in the total bacterial mass, an increase in the quantitative content of Lactobacillus spp., a decrease in the population of Bifidobacterium spp. and Bacteroides thetaiotaomicron.
CONCLUSIONS: The probiotic strain Enterococcus faecium L3 and the indigenous Enterococcus faecium do not have a significant effect on the metabolism of glucose and insulin, while they contribute to a similar change in the component composition of the microbiota and a decrease in the severity of gastroenterological complaints.
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##article.viewOnOriginalSite##About the authors
Natalya V. Bakulina
North-Western State Medical University named after I.I. Mechnikov
Email: natalya.bakulina@szgmu.ru
ORCID iD: 0000-0003-4075-4096
SPIN-code: 9503-8950
Scopus Author ID: 7201739080
ResearcherId: N-7299-2014
http://www.researcherid.com/rid/N-7299-2014
MD, Dr. Sci. (Med.)
Russian Federation, 47, Piskarevsky Ave., Saint Petersburg, 195067Sergey V. Tikhonov
North-Western State Medical University named after I.I. Mechniko
Email: sergeyvt2702@gmail.com
ORCID iD: 0000-0001-5720-3528
MD, Cand. Sci. (Med.), Assistant Professor
Russian Federation, 47, Piskarevsky Ave., Saint Petersburg, 195067Elena I. Ermolenko
Institute of Experimental Medicine
Email: lermolenko1@yandex.ru
ORCID iD: 0000-0002-2569-6660
SPIN-code: 9905-0590
Scopus Author ID: 13004322500
MD, Dr. Sci. (Med.), Professor
Russian Federation, Saint PetersburgMarina P. Kotyleva
Institute of Experimental Medicine
Email: mariha.lenivaya@mail.ru
ORCID iD: 0000-0003-1073-6508
Russian Federation, Saint Petersburg
Nadezhda S. Lavrenova
Institute of Experimental Medicine
Author for correspondence.
Email: nadezhda.lavrenova.vrn@gmail.com
ORCID iD: 0000-0003-0029-0741
SPIN-code: 9177-7815
Russian Federation, Saint Petersburg
Yulia G. Topalova
North-Western State Medical University named after I.I. Mechnikov
Email: topalova.julias@yandex.ru
ORCID iD: 0000-0003-3999-6848
SPIN-code: 1301-6443
MD
Russian Federation, 47, Piskarevsky Ave., Saint Petersburg, 195067Vladimir I. Simanenkov
North-Western State Medical University named after I.I. Mechnikov
Email: vi.simanenkov@mail.ru
ORCID iD: 0000-0002-1956-0070
SPIN-code: 8073-2401
MD, Dr. Sci. (Med.), Professor
Russian Federation, 47, Piskarevsky Ave., Saint Petersburg, 195067Alexander N. Suvorov
Institute of Experimental Medicine
Email: alexander_suvorov1@hotmail.com
ORCID iD: 0000-0002-2224-0019
SPIN-code: 8062-5281
MD, Dr. Sci. (Med.), Professor, Correspondence Member of the RAS
Russian Federation, Saint PetersburgReferences
- Ling C, Rönn T. Epigenetics in human obesity and type 2 diabetes. Cell Metab. 2019;29(5):1028–1044. doi: 10.1016/j.cmet.2019.03.009
- Koutnikova H, Genser B, Monteiro-Sepulveda M, et al. Impact of bacterial probiotics on obesity, diabetes and non-alcoholic fatty liver disease related variables: a systematic review and meta-analysis of randomised controlled trials. BMJ Open. 2019;9(3):e017995. doi: 10.1136/bmjopen-2017-017995
- Ridaura VK, Faith JJ, Rey FE, et al. Gut microbiota from twins discordant for obesity modulate metabolism in mice. Science. 2013;341(6150):1241214. doi: 10.1126/science.1241214
- Stanislawski MA, Dabelea D, Lange LA, et al. Gut microbiota phenotypes of obesity. NPJ Biofilms Microbiomes. 2019;5(1):18. doi: 10.1038/s41522-019-0091-8
- Ojo O, Feng QQ, Ojo OO, Wang XH. The role of dietary fibre in modulating gut microbiota dysbiosis in patients with type 2 diabetes: a systematic review and meta-analysis of randomised controlled trials. Nutrients. 2020;12(11):3239. doi: 10.3390/nu12113239
- Hill C, Guarner F, Reid G, et al. Expert consensus document. The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nat Rev Gastroenterol Hepatol. 2014;11(8):506–514. doi: 10.1038/nrgastro.2014.66
- Wang J, Tang H, Zhang C, et al. Modulation of gut microbiota during probiotic-mediated attenuation of metabolic syndrome in high fat diet-fed mice. ISME J. 2015;9(1):1–15. doi: 10.1038/ismej.2014.99
- Gomes AC, Bueno AA, de Souza RG, Mota JF. Gut microbiota, probiotics and diabetes. Nutr J. 2014;13:60. doi: 10.1186/1475-2891-13-60
- Ruan Y, Sun J, He J, et al. Effect of probiotics on glycemic control: a systematic review and meta-analysis of randomized, controlled trials. PLoS One. 2015;10(7):e0132121. doi: 10.1371/journal.pone.0132121
- Sun J, Buys NJ. Glucose- and glycaemic factor-lowering effects of probiotics on diabetes: a meta-analysis of randomised placebo-controlled trials. Br J Nutr. 2016;115(7):1167–1177. doi: 10.1017/S0007114516000076
- Giampieri F, Tulipani S, Alvarez-Suarez JM, et al. The strawberry: composition, nutritional quality, and impact on human health. Nutrition. 2012;28(1):9–19. doi: 10.1016/j.nut.2011.08.009
- Ejtahed HS, Mohtadi-Nia J, Homayouni-Rad A, et al. Probiotic yogurt improves antioxidant status in type 2 diabetic patients. Nutrition. 2012;28(5):539–543. doi: 10.1016/j.nut.2011.08.013
- Probiotics and prebiotics [Internet]. World Gastroenterology Organisation Global Guidelines. 2017. Available from: https://www.worldgastroenterology.org/guidelines/probiotics-and-prebiotics/probiotics-and-prebiotics-english. Accessed: Feb 23, 2022.
- Gonchar NV, Alyokhina LA, Suvorov AN. Probiotic strains of enterococci as a means of therapy and prevention of intestinal diseases in children (literature review). Experimental and clinical gastroenterology. 2013;(1):74–78. (In Russ.)
- Emaneini M, Hosseinkhani F, Jabalameli F, et al. Prevalence of vancomycin-resistant enterococcus in Iran: a systematic review and meta-analysis. Eur J Clin Microbiol Infect Dis. 2016;35(9):1387–1392. doi: 10.1007/s10096-016-2702-0
- Ermolenko EI, Voejkova AV, Suvorov AN. Influence of metabolites secreted by enterococcus cultures on the growth of mycoplasmas. Excerpt from VI Russian Congress of Infectious Disease Physicians, Russian Federation; Oct 29–31, 2003. Saint Petersburg; 2003. P. 72–73. (In Russ.)
- Averina OV, Ermolenko EI, Ratushny AYu, et al. Effect of probiotics on cytokine production in in vitro and in vivo systems. Medical immunology. 2015;17(5):443–454. (In Russ.). doi: 10.15789/1563-0625-2015-5-443-454
- Ermolenko EI. Lactic acid bacteria. Lambert academic Publishing, Deutschland Lambert; 2011. (In Russ.)
- Wu WC, Zhao W, Li S. Small intestinal bacteria overgrowth decreases small intestinal motility in the NASH rats. World J Gastroenterol. 2008;14(2):313–317. doi: 10.3748/wjg.14.313
- Il’in VK, Suvorov AN, Kiriukhina NV, et al. Autochthonous probiotics in prevention of infectious and inflammatory diseases of a human in the altered habitats. Vestn Ross Akad Med Nauk. 2013;68(2):56–62. (In Russ.). doi: 10.15690/vramn.v68i2.550
- Ermolenko EI, Yerofeev NP, Zakharova LB, et al. Influence of endogenous enterococci on microbiota. Features of the motor function of the colon in experimental dysbiosis. Zdorov’e – osnova chelovecheskogo potenciala: problemy i puti ikh resheniya =Health – the basis of human potential: problems and ways of their solution. 2016;11(2):769–781. (In Russ.)
- Donets VN. Possibilities of probiotic therapy of community-acquired pneumonia (clinical and experimental study) [dissertation]. Saint Petersburg; 2015. (In Russ.)
- Zeng Q, Li D, He Y, et al. Discrepant gut microbiota markers for the classification of obesity-related metabolic abnormalities. Sci Rep. 2019;9(1):13424. doi: 10.1038/s41598-019-49462-w
- Wang L, Alammar N, Singh R, et al. Gut microbial dysbiosis in the irritable bowel syndrome: a systematic review and meta-analysis of case-control studies. J Acad Nutr Diet. 2020;120(4):565–586. doi: 10.1016/j.jand.2019.05.015
- Liu R, Hong J, Xu X, et al. Gut microbiome and serum metabolome alterations in obesity and after weight-loss intervention. Nat Med. 2017;23(7):859–868. doi: 10.1038/nm.4358
- Bhattarai Y, Williams BB, Battaglioli EJ, et al. Gut microbiota-produced tryptamine activates an epithelial g-protein-coupled receptor to increase colonic secretion. Cell Host Microbe. 2018;23(6):775–785.e5. doi: 10.1016/j.chom.2018.05.004
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