Email this article Substitutional urethroplasty with tissue-engineered structures in an experiment
- Authors: Gorelova A.A.1,2, Murav’ev A.N.1,3, Vinogradova T.I.1, Gorelov A.I.2,4, Yudintseva N.M.5, Nashchekina Y.A.5, Samusenko I.A.6, Yablonsky P.K.1,2
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Affiliations:
- St. Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Healthcare of the Russian Federation
- St. Petersburg State University
- St. Petersburg Medico-Social Institute
- Pokrovskaya Municipal Hospital
- Institute of Cytology of the Russian Academy of Sciences
- Nikiforov Russian Center of Emergency and Radiation Medicine of the Ministry of Russian Federation for Civil Defense, Emergencies and Elimination of Consequences of Natural Disasters
- Issue: Vol 10, No 3 (2020)
- Pages: 201-208
- Section: Original articles
- URL: https://journals.rcsi.science/uroved/article/view/46031
- DOI: https://doi.org/10.17816/uroved46031
- ID: 46031
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Abstract
Introduction. In order to exclude the difficulties arising in the course of traditional surgical interventions in the scope of substitutional urethroplasty, in recent years, alternative materials have been developed using tissue engineering. This study is devoted to the development of new tissue-engineered constructions for urethroplasty using cells of different tissue origins and biopolymers, which is an urgent problem of modern medicine.
Aim. Experimental provision of a rationale for the possibility of using tissue-engineered constructions to replace urethral defects.
Methods and materials. Two-staged, experimental, controlled study. Dedicated to the development of tissue-engineered constructions (TEC) based on biopolymers seeded with mesenchymal stem cells or buccal epithelial cells. The prepared TECs were implanted into the rabbit’s urethra wall in a acute trauma model. Comparison of the results of the TECs usage with the “gold standard” – buccal urethraplasty was carried out.
Results. Urethrography showed similar results in all groups of animals, regardless the type of implanted material. No urethral patency was found, and confocal microscopy of urethral cryosections revealed the presence of nanoparticle-labeled mesenchymal stem cells / buccal epithelium cells with signs of their differentiation in the urothelial direction in the mucous layer.
Conclusion. The possibility of using tissue-engineered constructions based on biopolymers containing autologous mesenchymal stem cells or buccal epithelium cells for experimental substitutional urethroplasty was shown. The developed TECs can be used as an alternative to buccal urethroplasty in an experiment.
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##article.viewOnOriginalSite##About the authors
Anna A. Gorelova
St. Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Healthcare of the Russian Federation; St. Petersburg State University
Author for correspondence.
Email: gorelovauro@gmail.com
ORCID iD: 0000-0002-7010-7562
Postgraduate Student; Assistant-Professor, Department of Hospital Surgery
Russian Federation, Saint PetersburgAleksandr N. Murav’ev
St. Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Healthcare of the Russian Federation; St. Petersburg Medico-Social Institute
Email: urolog5@gmail.com
ORCID iD: 0000-0002-6974-5305
Candidate of Medical Science, Head of Urology, Gynecology and Abdominal Surgery Direction; Associate Professor, Department of Surgical Diseases
Russian Federation, Saint PetersburgTatiana I. Vinogradova
St. Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Healthcare of the Russian Federation
Email: vinogradova@spbniif.ru
ORCID iD: 0000-0002-5234-349X
Doctor of Medical Science, Professor, Chief Researcher
Russian Federation, Saint PetersburgAndrei I. Gorelov
St. Petersburg State University; Pokrovskaya Municipal Hospital
Email: gorelov_a_i@mail.ru
ORCID iD: 0000-0002-2858-5317
Doctor of Medical Science, Professor, Department of Hospital Surgery; Head, Urology Department
Russian Federation, Saint PetersburgNatalia M. Yudintseva
Institute of Cytology of the Russian Academy of Sciences
Email: yudintceva@mail.ru
ORCID iD: 0000-0002-7357-1571
Candidate of Biological Sciences, Senior Researcher
Russian Federation, Saint PetersburgYulia A. Nashchekina
Institute of Cytology of the Russian Academy of Sciences
Email: ulychka@mail.ru
ORCID iD: 0000-0002-4371-7445
Candidate of Biological Sciences, Research Fellow
Russian Federation, Saint PetersburgIgor A. Samusenko
Nikiforov Russian Center of Emergency and Radiation Medicine of the Ministry of Russian Federation for Civil Defense, Emergencies and Elimination of Consequences of Natural Disasters
Email: egors_2000@mail.ru
ORCID iD: 0000-0003-0622-3515
Candidate of Medical Science, Pathologist
Russian Federation, Saint PetersburgPetr K. Yablonsky
St. Petersburg State Research Institute of Phthisiopulmonology of the Ministry of Healthcare of the Russian Federation; St. Petersburg State University
Email: glhirurgb2@mail.ru
ORCID iD: 0000-0003-4385-9643
Doctor of Medical Science, Professor, Director; Head, Department of Hospital Surgery
Russian Federation, Saint PetersburgReferences
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