Email this article Effect of the complex of natural terpenes and vitamin E Renotinex® on free radical processes in patients with urolithiasis
- Authors: Streltsova O.S.1, Lyakhova A.A.1, Yashanova M.I.1, Kashina A.Y.1, Kalashnikov I.N.1, Pochtin D.P.2, Grebenkin E.V.3
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Affiliations:
- Privolzhskiy Research Medical University
- N.A. Semashko Nizhny Novgorod Regional Clinical Hospital
- LLC UNIM Laboratory
- Issue: Vol 13, No 1 (2023)
- Pages: 31-41
- Section: Original articles
- URL: https://journals.rcsi.science/uroved/article/view/134241
- DOI: https://doi.org/10.17816/uroved117549
- ID: 134241
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Abstract
BACKGROUND: One of the leading links in the pathogenesis of urolithiasis is oxidative stress, which occurs as a result of the intensification of free radical oxidation with insufficient activity of the antioxidant defense system of the body.
AIM: The aim of the study was to study the effect of a complex of natural terpenes and vitamin E Renotineх® on the state of oxidative stress in patients with urolithiasis in the postoperative period after percutaneous nephrolithotripsy.
MATERIALS AND METHODS: The study included 42 patients. Of the 35 people who underwent percutaneous nephrolithotripsy, 2 groups were formed. Patients of the main group (n = 20), in contrast to the control group (n = 15), received Renotinex® (Polaris LLC, Russia) before and after surgery, 2 capsules (1 capsule 300 mg) twice a day. To determine the main indicators of the activity of free-radical processes in the body, all patients underwent a blood test on the 3rd day after surgery. The total activity of the pro- and antioxidant systems in blood plasma was determined by the method of induced chemiluminescence. The activity of lipid peroxidation was assessed by determining the concentration of active products of thiobarbituric acid — secondary products of lipid peroxidation in blood erythrocytes. The activity of antioxidant enzymes was studied by spectrophotometric method. To determine the ability of blood plasma to crystallize in some of the same patients with urolithiasis while taking Renotinex®, the method of wedge dehydration was used. For a comparative analysis of the crystal structure of dried blood plasma samples (facies), 7 healthy volunteers were included in the study, which made up the third group (comparison group).
RESULTS: The results of the study reliably demonstrate that the composition of the complex of natural terpenes and vitamin E Renotinex® has a positive effect on free-radical processes in patients with urolithiasis, biochemical parameters of blood plasma (indicators of induced chemiluminescence, catalase activity, malondialdehyde level), as well as on crystallization processes blood plasma, which is confirmed by the method of wedge dehydration of blood plasma. Any change in the body caused by excessive activity of free-radical oxidation processes is reflected in the composition of the blood, and specifically in the indicators of the “mechanical” characteristics of blood plasma (viscosity, structure, surface tension), which determine the shape of the resulting structures of the drying drop of biological fluid.
CONCLUSIONS: The complex of natural terpenes and vitamin E Renotinex® has a positive effect on free-radical processes, biochemical parameters of blood plasma, as well as on the systemic structural organization of blood plasma in patients operated on for urolithiasis. The inclusion of the combined product Renotinex® in the treatment regimen for patients with urolithiasis is pathogenetically justified.
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##article.viewOnOriginalSite##About the authors
Olga S. Streltsova
Privolzhskiy Research Medical University
Email: strelzova_uro@mail.ru
ORCID iD: 0000-0002-9097-0267
SPIN-code: 9674-0382
Dr. Sci. (Med.), professor of the E.V. Shakhov Department of Urology
Russian Federation, Nizhny NovgorodAnastasiya A. Lyakhova
Privolzhskiy Research Medical University
Email: anastasyalyakhova@yandex.ru
SPIN-code: 2284-6849
assistant of the Department of Biology
Russian Federation, Nizhny NovgorodMariya I. Yashanova
Privolzhskiy Research Medical University
Email: yammi2006@mail.ru
SPIN-code: 1556-6456
assistant of the Department of Biology
Russian Federation, Nizhny NovgorodAnna Yu. Kashina
Privolzhskiy Research Medical University
Email: kasanyutka@yandex.ru
SPIN-code: 1381-1460
laboratory assistant of the Department of Biology
Russian Federation, Nizhny NovgorodIlya N. Kalashnikov
Privolzhskiy Research Medical University
Email: ilia17ne@mail.ru
SPIN-code: 2926-1486
Cand. Sci. (Biol.), head of the Department of Biology
Russian Federation, Nizhny NovgorodDmitry P. Pochtin
N.A. Semashko Nizhny Novgorod Regional Clinical Hospital
Author for correspondence.
Email: dpochtin@mail.ru
ORCID iD: 0000-0003-4634-408X
urologist
Russian Federation, Nizhny NovgorodEvgenii V. Grebenkin
LLC UNIM Laboratory
Email: grebenkin_urolog@mail.ru
ORCID iD: 0000-0002-4990-6722
SPIN-code: 6798-3427
Cand. Sci. (Med.), pathologist
Russian Federation, MoscowReferences
- Gudenko YuA, Kazanskaya IV, Lobzhanidze ZD. Rovatinex usage in pediatric urology. Experimental and Clinical Urology. 2013;(3): 61–65. (In Russ.)
- Apolikhin OI, Sivkov AV, Komarova VA, et al. Urolithiasis in the Russian Federation (2005–2016). Experimental and Clinical Urology. 2018;(4):4–14. (In Russ.) doi: 10.29188/2222-8543-2022-15-2-10-17
- Heers H, Turney B. Trends in urological stone disease: A 5-year update of hospital episode statistics. BJU Int. 2016;118(5):785–789. doi: 10.1111/bju.13520
- Malkhasyan VA, Ivanov VYu, Khodyreva LA, et al. Analysis of specialized medical care to patients with renal colic in Moscow urological hospitals. Experimental and Clinical Urology. 2016;(4):18–25. (In Russ.)
- Gadzhiyev NK, Brovkin SS, Grigor’yev VE, et al. Metaphylaxis of urolithiasis: new look, modern approach, implementation in a mobile application. Urologiia. 2017;(1):124–129. (In Russ.) doi: 10.18565/umL2017.L124-129
- Tolordava ER. Rol bakterialnykh bioplenok v etiopatogeneze mochekamennoi bolezni [dissertation abstract]. Moscow; 2014. 27 p.
- Liu YQ, Lu J, Hao YC. Predicting model based on risk factors for urosepsis after percutaneous nephrolithotomy. Beijing Da Xue Xue Bao Yi Xue Ban. 2018;50(3):507–513.
- Koras O, Bozkurt IH, Yonguc T. Risk factors for postoperative infectious complications following percutaneous nephrolithotomy: a prospective clinical study. Urolithiasis. 2015;43(1):55–60. doi: 10.1007/s00240-014-0730-8
- Kamalov AA, Okhobotov DA, Nizov AN, et al. The role of oxidative stress in the pathogenesis of calcium oxalate urolithiasis. Russian Medical Journal. 2019;(11):2–6. (In Russ.)
- Alyayev YuG, Glybochko PV, Pushkar’ DYu. Urologiya: rossiyskie klinicheskie rekomendatsii. Moscow: Medforum; 2017. 544. p. (In Russ.)
- Skolarikos A, Neisius A, Petrik A, et al. EAU Guidelines on Urolithiasis. European Association of Urology. 2022 [cited 2023 March 01]. Available from: https://uroweb.org/guidelines/urolithiasis
- Kuz’mina EI, Nelyubin AS, Shchennikova MK. Primenenie indutsirovannoi khemilyuminestsentsii dlya otsenok svobodnoradikal’nykh reaktsii v biologicheskikh substratakh. Mezhvuzovskii sbornik Biokhimiya i biofizika mikroorganizmov. Gor’kii. 1983. P. 41–43. (In Russ.)
- Arutyunyan AV, Dubinina YeE, Zybina NN. Metody otsenki svobodnoradikal’nogo okisleniya i antioksidantnoi sistemy organizma. Metodicheskie rekomendatsii. Saint Petersburg: IKF Foliant; 2000. 104 p. (In Russ.)
- Shatokhina SN, Shabalin VN. Morfologiya biologicheskikh zhidkostey — novoye napravleniye v klinicheskoy meditsine. Almanac of Clinical Medicine. 2003;(6):404–420. (In Russ.)
- Streltsova OS, Vlasov VV, Grebenkin EV, Controlled fragmentation of urinary stones as a method of preventing inflammatory infections in the treatment of urolithiasis (experience in successful clinical use). Sovremennye Tehnologii v Medicine. 2021;13(3):55–63. (In Russ.) doi: 10.17691/stm2021.13.3.07
- Misharina TA, Alinkina ES, Fatkulina LD, et al. Influence of the composition of essential oils on their antioxidant and antiradical properties. Applied Biochemistry and Microbiology. 2012;48(1): 102–107. (In Russ.)
- Misharina TA, Terenina MB, Krikunova NI. Antioxidant properties of essential oils. Applied Biochemistry and Microbiology. 2009;45(6):642–647 (In Russ.)
- Sieniawska E, Swatko-Ossor M, Sawicki R. Natural Terpenes Influence the Activity of Antibiotics against Isolated Mycobacterium tuberculosis. Med Princ and Pract. 2017;26(2):108–112. doi: 10.1159/000454680
- Shaderkina VA, Shaderkin IA. Terpenes and their application in clinical practice. Experimental and Clinical Urology. 2019;(1):77–80. (In Russ.) doi: 10.29188/2222-8543-2019-11-1-77-80
- Han Y. Ginkgo terpene component has an anti-inflammatory effect on Candida albicans — caused arthritic inflammation. Int Immunopharmacol. 2005;5(6):1049–1056. doi: 10.1016/j.intimp.2005.02.002
- Chua ME, Park JH, Castillo JC. Terpene compound drug as medical expulsive therapy for ureterolithiasis: a meta-analysis. Urolithiasis. 2013;41(2):143–151. doi: 10.1007/s00240-012-0538-3
- Uzbekov MG. Lipid peroxidation and antioxidant systems in mental disorders. Communication IV. Social and Clinical Psychiatry. 2016;26(3):65–71. (In Russ.)
- Telesheva LF, Kazmirchuk AV, Bychkovskikh VA, et al. Influence of bestim immunomodulator on immune status and free-radical oxidation in patients with chronic pyelonephritis associated with urolithiasis. Human. Sport. Medicine. 2016;16(2):5–17. (In Russ.) doi: 10.14529/hsm160201
- Ship SA, Ratnikova LI. Free radical oxidative processes in the organism. Izvestiya Vysshikh Uchebnykh Zavedeniy. Ural’skiy Region. 2015;(2):105–109. (In Russ.)
- Nikerova KM. Aktivnost’ fermentov antioksidantnoi sistemy pri izmenenii stsenariev ksilogeneza u Betula pendula Roth i Pinus sylvestris [dissertation]. Petrozavodsk; 2020. 201 p. (In Russ.)
- Mirovich VM, Privalova EG, FGBOU VO Irkutskii gosudarstvennyi meditsinskii universitet MZ RF, kafedra farmakognozii i farmatsevticheskoi tekhnologii Biologicheski aktivnye veshchestva rastenii (polisakharidy, efirnye masla, fenologlikozidy, kumariny, flavonoidy). Uchebnoe posobie. Irkutsk: IGMU; 2018. 70 p. (In Russ.)
- Polyakova LV, Yashanova MI, Strel’tsova OS, et al. Influence of the treatment with using the terpene drug on the activity of antioxidant enzymes in erythrocytes of patients with acute urolithiasis In: XXV Nizhny Novgorod session of young scientists (technical, natural, humanitarian sciences): materials of theses and reports. 10–13 November 2020. Nizhnii Novgorod: NRL. P. 203–205. (In Russ.)
- Bird VY, Khan SR. How do stones form? Is unification of theories on stone formation possible? Arch Esp Urol. 2017;70(1):12–27.
- Prakhin YeI, Evert LS, Borozdun SV, et al. The role of nutrition in formation of oxalate-calcium crystalluria and nephrolithiasis in childhood. Pediatric Nutrition. 2003;1(2):64–66. (In Russ.)
- Streltsova OS, Krupin VN, Lazukin VF, et al. Prevalence and predictors of incidence of urolithiasis in a representative region. Urology Reports (St. Petersburg). 2022;12(2):105–116. (In Russ.) doi: 10.17816/uroved105423
- Skugoreva SG, Ashikhmina TYA, Fokina AI. Chemical grounds of toxic effect of heavy metals (review). Theoretical and Applied Ecology. 2016;(1):4–13. (In Russ.) doi: 10.25750/1995-4301-2016-1-014-019
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