Email this article The use of cellular technologies in the treatment of children with congenital cleft palate
- Authors: Stepanova Y.V.1, Tsyplakova M.S.1, Usoltseva A.S.1, Enukashvili N.I.2,3, Bagaeva V.V.2, Semyonov M.G.4, Murashko T.V.1, Ponamareva K.G.5
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Affiliations:
- The Turner Scientific Research Institute for Children’s Orthopedics
- Pokrovsky Bank of Stem Cells
- Institute of Cytology of RAS
- North-Western State Medical University n. a. I.I. Mechnikov
- Saint Petersburg State University
- Issue: Vol 5, No 4 (2017)
- Pages: 31-37
- Section: Articles
- URL: https://journals.rcsi.science/turner/article/view/7671
- DOI: https://doi.org/10.17816/PTORS5431-37
- ID: 7671
Cite item
Abstract
Background. Mesenchymal stromal cells (MSCs) are multipotent stem cells capable of differentiation in the osteogenic, chondrogenic, and adipogenic directions that are widely used in the development of new cellular biomedical technologies.
Aim. We investigated the effect of MSCs on osteogenesis in the congenital defect of the alveolar process of the upper jaw with the aim of improving the treatment results for children with congenital cleft palate.
Materials and methods. At the department of maxillofacial surgery of the Turner Institute for Children’s Orthopedics, 46 patients with a diagnosis of congenital cleft palate were observed in 2017. Six patients with congenital cleft palate in the region of the defect of the hard palate and the alveolar process of the upper jaw underwent uranoplasty and implantation of a mixture (1 : 4) of MSCs and preosteocytes derived from them on the osteogenic membrane. The control group consisted of 40 age-matched patients who underwent the same surgery but without the use of MSCs. The distance between the cleaved portions of the alveolar process of the upper jaw ranged from 0.5–1.0 cm. The follow-up period was 6–9 months.
Results. On X-ray examination 6–9 months after the operation in the bone defect area and implantation of MSCs in all patients, tissue with a density corresponding to that of bone was found. In the control group, bone tissue was not formed in the diastasis of the alveolar bone. There were no significant differences in the timing of wound healing and course of the postoperative period.
Сonclusion. Tissue engineering helped in the treatment of severe congenital malformations of the maxillofacial area. There are good prospects for using MSCs in the surgical treatment of defects of the facial skeleton.
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##article.viewOnOriginalSite##About the authors
Yulia V. Stepanova
The Turner Scientific Research Institute for Children’s Orthopedics
Author for correspondence.
Email: travmaortoped@mail.ru
MD, PhD, chief of the Department of Maxillofacial Surgery
Russian Federation, 64, Parkovaya str., Saint-Petersburg, Pushkin, 196603Margarita S. Tsyplakova
The Turner Scientific Research Institute for Children’s Orthopedics
Email: travmaortoped@mail.ru
MD, PhD, senior research associate of Department of Maxillofacial Surgery
Russian Federation, 64, Parkovaya str., Saint-Petersburg, Pushkin, 196603Anna S. Usoltseva
The Turner Scientific Research Institute for Children’s Orthopedics
Email: travmaortoped@mail.ru
MD, maxillofacial surgeon of the Department of Maxillofacial Surgery
Russian Federation, 64, Parkovaya str., Saint-Petersburg, Pushkin, 196603Natela I. Enukashvili
Pokrovsky Bank of Stem Cells; Institute of Cytology of RAS
Email: travmaortoped@mail.ru
PhD in Biological Sciences, senior researcher of Laboratory of Cell Morphology of the Institute of Cytology of RAS; head of the Research and Development Department, Pokrovsky Bank of Stem Cells
Russian Federation, Saint Petersburg; 4, Tikhoretsky street, St.Petersburg, 194064Varvara V. Bagaeva
Pokrovsky Bank of Stem Cells
Email: travmaortoped@mail.ru
specialist of the Research and Development Department
Russian Federation, Saint PetersburgMikhail G. Semyonov
North-Western State Medical University n. a. I.I. Mechnikov
Email: travmaortoped@mail.ru
MD, PhD, рrofessor, head of the Department of Maxillofacial Surgery and Surgical Dentistry n.a. A.A. Limberg, North-Western State Medical University n.a. I.I. Mechnikov
Russian Federation, 41, Kirochnaya street, Saint-Petersburg, 191015Tatiana V. Murashko
The Turner Scientific Research Institute for Children’s Orthopedics
Email: travmaortoped@mail.ru
MD, radiologist
Russian Federation, 64, Parkovaya str., Saint-Petersburg, Pushkin, 196603Karina G. Ponamareva
Saint Petersburg State University
Email: travmaortoped@mail.ru
MD, PhD, associate professor of the Department of Stomatology, Faculty of Stomatology and Medical Technology
Russian Federation, 7/9, Universitetskaya nab., St.Petersburg, 199034References
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