Features of the formation of early immunological tolerance during immunotherapy with allergens in patients with allergic rhinitis
- 作者: Volkova D.1, Bikchantaeva G.2, Luntsov A.2, Bareycheva O.2, Umarova N.3, Skorokhodkina O.1
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隶属关系:
- Kazan State Medical University
- Republican Clinical Hospital of the Republic of Tatarstan Ministry of Health
- Kazan National Research Technological University
- 期: 卷 21, 编号 2 (2024)
- 页面: 230-242
- 栏目: Original studies
- URL: https://journals.rcsi.science/raj/article/view/260058
- DOI: https://doi.org/10.36691/RJA16932
- ID: 260058
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详细
BACKGROUND: The effectiveness of allergen-specific immunotherapy has been proven in studies. Studying the level and expanded spectrum of Th1, Th2, Treg and Breg cytokines against the background of allergen-specific immunotherapy, as well as allergen-specific IgG4 (asIgG4), is promising, which will clarify the mechanisms of the effectiveness of allergen-specific immunotherapy in patients with allergic rhinitis.
AIM: To evaluate the clinical effectiveness of allergen-specific immunotherapy in patients with allergic rhinitis and to identify the most significant biomarkers of the formation of early immunological tolerance.
MATERIALS AND METHODS: We examined 30 patients with allergic rhinitis aged 19 to 60 years who received allergen-specific immunotherapy. The clinical effectiveness was assessed using the validated Combined Symptom Medication Score (CSMS). All patients had their serum levels of IL-4, IL-13, IFN-γ, IL-12, IL-10, TGF-β, and asIgG4 of enzyme-linked immunosorbent assay recorded at baseline and 6 months after the start of allergen-specific immunotherapy.
RESULTS: Allergen-specific immunotherapy has been demonstrated to be highly clinically effective in achieving control of allergic rhinitis. At baseline, the total CSMS score was 3.75 [3.33; 4.5], at the end of 6 months ― 1.83 [1.17; 2.67] (p=0.000002). When analyzing the dynamics of the cytokine profile and asIgG4, an increasing in the level of TGF-β (p=0.000002), IL-12 (p=0.000002) and asIgG4 (p=0.000003) was revealed, as well as a decreasing in the level of IL-4 (p=0.000024) 6 months after the start of allergen-specific immunotherapy. We did not register an increase in IFN-γ and the expected increase in IL-10 and decrease of IL-13. The correlation and principal component analysis showed that the clinical effect of allergen-specific immunotherapy was associated with an increase in TGF-β and IL-12, a moderate increase in asIgG4 with a decrease of IL-4.
CONCLUSION: The results demonstrate the high clinical effectiveness of allergen-specific immunotherapy within 6 months from the moment of its initiation. Significant marker of the effectiveness of allergen-specific immunotherapy at the stage of early tolerance formation is TGF-β, which increasement was in correlation with an increased IL-12 with a decrease of IL-4. The increase in asIgG4 is less pronounced. The conducted correlation and principal component analysis showed the relationship of the discussed biomarkers with the CSMS, which may indicate in favor of their choice as potential biomarkers for assessing the effectiveness of allergen-specific immunotherapy.
作者简介
Daria Volkova
Kazan State Medical University
编辑信件的主要联系方式.
Email: volkdash190296@gmail.com
ORCID iD: 0000-0001-5262-8420
SPIN 代码: 2030-1753
俄罗斯联邦, Kazan
Gulnara Bikchantaeva
Republican Clinical Hospital of the Republic of Tatarstan Ministry of Health
Email: grlki564@yandex.ru
ORCID iD: 0009-0006-5066-814X
SPIN 代码: 2216-5197
俄罗斯联邦, Kazan
Alexey Luntsov
Republican Clinical Hospital of the Republic of Tatarstan Ministry of Health
Email: luntsov@gmail.com
ORCID iD: 0000-0003-2552-2107
SPIN 代码: 7076-1967
MD, Cand. Sci. (Med.)
俄罗斯联邦, KazanOlga Bareycheva
Republican Clinical Hospital of the Republic of Tatarstan Ministry of Health
Email: olga-alex21@mail.ru
ORCID iD: 0000-0002-1419-8746
SPIN 代码: 8728-8883
俄罗斯联邦, Kazan
Nailya Umarova
Kazan National Research Technological University
Email: nailyaumarova@yandex.ru
ORCID iD: 0000-0002-4284-6034
SPIN 代码: 5597-2620
Cand. Sci. (Chem.), Assistant Professor
俄罗斯联邦, KazanOlesya Skorokhodkina
Kazan State Medical University
Email: olesya-27@rambler.ru
ORCID iD: 0000-0001-5793-5753
SPIN 代码: 8649-6138
MD, Dr. Sci. (Med.), Professor
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