Study of the silver birchpollen allergoid
- 作者: Kulaga O.1, Avoyan G.1, Esaulova D.1, Andreev I.1, Nechay K.1, Andreev A.1, Kicheeva K.2, Baklakova O.3, Mislavsky O.1, Gegechkori V.2, Cherchenko N.1, Sankov M.1, Toptygin А.1, Shvets S.1, Romanova T.1, Latysheva E.1, Martynov А.1, Khaitov M.1,4
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隶属关系:
- National Research Center ― Institute of Immunology
- The First Sechenov Moscow State Medical University (Sechenov University)
- Academy of Postgraduate Education of the Federal Research and Clinical Center of Specialized Medical Care and Medical Technologies
- N.I. Pirogov Russian National Research Medical University
- 期: 卷 19, 编号 3 (2022)
- 页面: 328-335
- 栏目: Original studies
- URL: https://journals.rcsi.science/raj/article/view/121748
- DOI: https://doi.org/10.36691/RJA1549
- ID: 121748
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BACKGROUND: Birch pollen is one of the main allergens in central Russia, Europe, North America, and several other regions. Allergens contained in birch pollen often cause immunoglobulin E cross-reactivity with homologous proteins both in other trees and in various foods. Allergen immunotherapy is currently the only allergy-treating method that directly affects various links in disease pathogenesis, thereby changing the body’s response to the action of the allergen. The clinical effectiveness of allergen immunotherapy reaches 80%–90% and is expressed by decreased clinical manifestations and the need for drugs. An allergoid, unlike an allergen, retains its immunogenicity, but significantly loses its allergenicity, which makes it safer for use with allergen immunotherapy.
AIM: To obtain an allergoid from the pollen of silver birch (Bétula péndula) by processing it with glutaraldehyde.
MATERIALS AND METHODS: The purified allergen isolated from pollen, by degreasing and water–salt extraction, was dissolved in phosphate-buffered saline at 7.5 pH and polymerized in 0.1% of glutaraldehyde solution. Stabilization was conducted with a sodium borohydride solution.
RESULTS: The allergoid significantly reduced its allergenicity compared to the original allergen after assessing the specific activity using a competitive enzyme-linked immunosorbent assay.
CONCLUSIONS: This study obtained an allergen of the silver birch. The resulting allergen contains major and minor antigens of silver birch (Bet v 1, Bet v 2, Bet v 3, Bet v 6, and Bet v 7). Based on these, an allergoid was obtained by polymerization with glutaraldehyde. The resulting allergoid has a large molecular weight and a low allergenic activity, in comparison with the original allergen. Thereby, this allergoid can become the basis for obtaining new allergen immunotherapy preparations.
作者简介
Olga Kulaga
National Research Center ― Institute of Immunology
Email: olga.surova.94@mail.ru
ORCID iD: 0000-0003-1675-3691
SPIN 代码: 4736-2614
俄罗斯联邦, Moscow
Gayane Avoyan
National Research Center ― Institute of Immunology
Email: avoyan.gayane@gmail.com
ORCID iD: 0000-0003-4428-0801
SPIN 代码: 7196-4339
俄罗斯联邦, Moscow
Daria Esaulova
National Research Center ― Institute of Immunology
Email: esaulova.d.r@gmail.com
ORCID iD: 0000-0003-0283-5637
SPIN 代码: 4399-8631
MD
俄罗斯联邦, MoscowIgor Andreev
National Research Center ― Institute of Immunology
编辑信件的主要联系方式.
Email: iva66@list.ru
ORCID iD: 0000-0001-6162-6726
SPIN 代码: 8072-9669
MD, Cand. Sci. (Med.)
俄罗斯联邦, MoscowKsenia Nechay
National Research Center ― Institute of Immunology
Email: xenya.ne4ay2016@yandex.ru
ORCID iD: 0000-0001-6052-9721
SPIN 代码: 7206-6660
俄罗斯联邦, Moscow
Alexandr Andreev
National Research Center ― Institute of Immunology
Email: cahek_ahdreeb@mail.ru
ORCID iD: 0000-0002-6257-6289
SPIN 代码: 7126-4748
俄罗斯联邦, Moscow
Karina Kicheeva
The First Sechenov Moscow State Medical University (Sechenov University)
Email: kicheeva2001@yandex.ru
ORCID iD: 0000-0003-3328-1403
俄罗斯联邦, Moscow
Olga Baklakova
Academy of Postgraduate Education of the Federal Research and Clinical Center of Specialized Medical Care and Medical Technologies
Email: olbakserg@gmail.com
ORCID iD: 0000-0002-0066-3096
SPIN 代码: 8424-2189
MD
俄罗斯联邦, MoscowOleg Mislavsky
National Research Center ― Institute of Immunology
Email: mislavsky.oleg@yandex.ru
ORCID iD: 0000-0002-9996-5050
SPIN 代码: 6862-2135
Cand. Sci. (Pharm)
俄罗斯联邦, MoscowVladimir Gegechkori
The First Sechenov Moscow State Medical University (Sechenov University)
Email: gegechkori_v_i@staff.sechenov.ru
ORCID iD: 0000-0001-8437-1148
SPIN 代码: 8964-0804
Cand. Sci. (Pharm), Assistant Professor
俄罗斯联邦, MoscowNikolay Cherchenko
National Research Center ― Institute of Immunology
Email: cherchenk-o@mail.ru
ORCID iD: 0000-0001-5714-0045
SPIN 代码: 4437-9547
Cand. Sci. (Biol.)
俄罗斯联邦, MoscowMikhail Sankov
National Research Center ― Institute of Immunology
Email: sankov_m54@mail.ru
ORCID iD: 0000-0003-4969-1626
SPIN 代码: 4258-1796
Cand. Sci. (Biol.)
俄罗斯联邦, MoscowАndrey Toptygin
National Research Center ― Institute of Immunology
Email: atop2007@yandex.ru
ORCID iD: 0000-0003-4232-0670
SPIN 代码: 5282-9472
MD, Cand. Sci. (Med.)
俄罗斯联邦, MoscowSvetlana Shvets
National Research Center ― Institute of Immunology
Email: smshvets@gmail.com
ORCID iD: 0000-0001-9642-4016
SPIN 代码: 9954-8252
MD, Cand. Sci. (Med.)
俄罗斯联邦, MoscowTatiana Romanova
National Research Center ― Institute of Immunology
Email: ts_romanova@mail.ru
ORCID iD: 0000-0003-3350-3811
SPIN 代码: 8027-8625
MD, Cand. Sci. (Med.)
俄罗斯联邦, MoscowElena Latysheva
National Research Center ― Institute of Immunology
Email: ea.latysheva@nrcii.ru
ORCID iD: 0000-0002-1606-205X
SPIN 代码: 2063-7973
MD, Dr. Sci. (Med.)
俄罗斯联邦, MoscowАlexander Martynov
National Research Center ― Institute of Immunology
Email: immune48@mail.ru
ORCID iD: 0000-0001-9761-8058
SPIN 代码: 5829-5580
MD, Cand. Sci. (Med.)
俄罗斯联邦, MoscowMusa Khaitov
National Research Center ― Institute of Immunology; N.I. Pirogov Russian National Research Medical University
Email: mr.khaitov@nrcii.ru
ORCID iD: 0000-0003-4961-9640
SPIN 代码: 3199-9803
MD, Dr. Sci. (Med.), Professor, Corresponding Member of the Russian Academy of Sciences
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