Assessment of cytokine profile and T-lymphocyte subpopulations in blood serum in children with allergic diseases of the respiratory system
- Authors: Prosekova EV1, Turyanskaya AI1, Plekhova NG1, Dolgopolov MS1, Sabynych VA1
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Affiliations:
- Federal State Budgetary Educational Institution of Higher Education «Pacific State Medical University» of the Ministry of Healthcare of the Russian Federation
- Issue: Vol 16, No 3 (2019)
- Pages: 52-60
- Section: Articles
- URL: https://journals.rcsi.science/raj/article/view/121848
- DOI: https://doi.org/10.36691/RJA1210
- ID: 121848
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Abstract
Expansion of the range of examined T-helper clones has determined more complex immune mechanisms for the implementation of allergic inflammation. Objective. To characterize the parameters and relationships between the serum cytokine profile and T-lympho-cyte subpopulation in peripheral blood of children with bronchial asthma and allergic rhinitis. Materials and methods. 150 children aged between 3-11 years old with bronchial asthma, and allergic rhinitis and 30 healthy volunteers were examined. Immunological parameters were assessed by flow cytometry, the concentration of serum interleukins and IgE were determined by means of enzymelinked immunosorbent assay. Statistical analysis was performed with «Statistica 10» program with a critical level of significance p<0.05. Results. High levels of interleukins -4, -8, -13, 17A were determined, IL-7F level was not significantly different from that in control group and low level of IFN-y was found in the serum of children with allergic diseases. The number of CD3+CD8+CD45RO+, CD3+CD8+CD45RACD45RO+ T-lymphocytes, CD3+CD4+ T-helper cells and Th17 were increased and at the same time CD3+CD4+CD45RO+ memory cells were decreased In bronchial asthma and allergic rhinitis children. Number of Th17 cells in healthy children was 9.49±1.6%, in allergic children it was significantly higher - 14.5±0.77% (p<0.001). Analyses of serum cytokine count in children with isolated BA and in association with allergic rhinitis revealed multidirectional correlations differing in strength and direction from those in the group of healthy children. Conclusion. In children with isolated bronchial asthma and associated with allergic rhinitis the following parameters were found: CD3+CD4+ T-cells count was comparable to that in healthy children, the imbalance of T-helper subpopulation: prevalence of Th2 and Th17, activation of IL-17A, IL-4, IL-8, IL-13 synthesis and low level of serum IFN-y.
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##article.viewOnOriginalSite##About the authors
E V Prosekova
Federal State Budgetary Educational Institution of Higher Education «Pacific State Medical University» of the Ministry of Healthcare of the Russian Federation
Email: pros.ev@mail.ru
доктор медицинских наук, профессор, зав. кафедрой клинической лабораторной диагностики, общей и клинической иммунологии
A I Turyanskaya
Federal State Budgetary Educational Institution of Higher Education «Pacific State Medical University» of the Ministry of Healthcare of the Russian Federationассистент кафедры клинической лабораторной диагностики, общей и клинической иммунологии
N G Plekhova
Federal State Budgetary Educational Institution of Higher Education «Pacific State Medical University» of the Ministry of Healthcare of the Russian Federationдоктор биологических наук, профессор кафедры клинической лабораторной диагностики, общей и клинической иммунологии, руководитель ЦНИЛ
M S Dolgopolov
Federal State Budgetary Educational Institution of Higher Education «Pacific State Medical University» of the Ministry of Healthcare of the Russian Federationассистент кафедры клинической лабораторной диагностики, общей и клинической иммунологии
V A Sabynych
Federal State Budgetary Educational Institution of Higher Education «Pacific State Medical University» of the Ministry of Healthcare of the Russian Federationкандидат медицинских наук, доцент кафедры клинической лабораторной диагностики, общей и клинической иммунологии
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