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Post a Comment Cerebral damage pathogeny peculiarities in local and globalcerebral perfusion impairment models in rats
- Authors: Zelenenko M.A.1, Pechatnikova V.A.2, Zaplutanov V.A.1, Tsygan N.V.3, Verlov N.A.2, Khotin M.G.4, Vasiliev A.G.5, Trashkov A.P.2
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Affiliations:
- I.M. Sechenov institute of evolutionary physiology and biochemistry, Russian academy of sciences
- Petersburg Nuclear Physics Institute named by B.P. Konstantinov
- Military Medical Academy n.a. S.M. Kirov
- Institute of Cytology, Russian academy of sciences
- St. Petersburg State Pediatric Medical University
- Issue: Vol 8, No 6 (2017)
- Pages: 62-71
- Section: Articles
- URL: https://journals.rcsi.science/pediatr/article/view/7878
- DOI: https://doi.org/10.17816/PED8662-71
- ID: 7878
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Abstract
A complex study of clinical and laboratory pathogenic features of cerebrovascular disease has been performed in rats using two experimental models: local ischemia with cerebral infarction and global ischemia caused by acute or chronic impairment of cerebral circulation without verified site of brain damage. A pronounced difference has been demonstrated between the two studied models of cerebral impairment. The development of brain damage site constituting 20-30% of the total cortex of the impaired hemisphere was demonstrated to be accompanied by a considerable increase of basic nervous tissue damage markers (neuron-specific enolase, S100β-protein), development of endothelial dysfunction and hemostasiological disturbances. Irreparable cerebral circulation impairment by ligation of carotid arteries was followed by considerably lower lethality in the experimental animals, neurospecific markers’ moderate dynamics but however side by side with pronounced and inadequately compensated manifestations in the hemostasis system and vascular endothelium caused by brain hypoxia.
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##article.viewOnOriginalSite##About the authors
Maria A. Zelenenko
I.M. Sechenov institute of evolutionary physiology and biochemistry, Russian academy of sciences
Author for correspondence.
Email: magu56110@gmail.com
MD, researcher, Experimental Pharmacology Dept
Russian Federation, Saint PetersburgValeria A. Pechatnikova
Petersburg Nuclear Physics Institute named by B.P. Konstantinov
Email: floluttrell@gmail.com
researcher Radiopharmpreparations Test Center
Russian Federation, GatchinaVassiliy A. Zaplutanov
I.M. Sechenov institute of evolutionary physiology and biochemistry, Russian academy of sciences
Email: vasiliy@zaplutanov.ru
MD, researcher, Experimental Pharmacology Deptartment
Russian Federation, Saint PetersburgNikolay V. Tsygan
Military Medical Academy n.a. S.M. Kirov
Email: 77tn77@gmail.com
MD, PhD, Dr.Med. Sci., Assoc. Prof. Neurology Deptartment
Russian Federation, Saint PetersburgNikolay A. Verlov
Petersburg Nuclear Physics Institute named by B.P. Konstantinov
Email: virlov@gmail.com
PhD, Senior researcher Radiopharmpreparations Test Center
Russian Federation, GatchinaMichael G. Khotin
Institute of Cytology, Russian academy of sciences
Email: h_mg@mail.ru
PhD, Heaqd, Lab. Of Biomedical Technologies and Tests
Russian Federation, Saint PetersburgAndrei G. Vasiliev
St. Petersburg State Pediatric Medical University
Email: avas7@mail.ru
MD, PhD, Dr. Med. Sci., Head of Pathophysiology Deptartment
Russian Federation, Saint PetersburgAlexander P. Trashkov
Petersburg Nuclear Physics Institute named by B.P. Konstantinov
Email: alexandr.trashkov@gmail.com
MD, PhD, Head Radiopharmpreparations Test Center
Russian Federation, GatchinaReferences
- Васильев А.Г., Хайцев Н.В., Трашков А.П. Практикум по патофизиологии: учебное пособие / под редакцией проф. А.Г. Васильева, Н.В. Хайцева. — СПб., 2014. [Vasil’ev AG, Khaytsev NV, Trashkov AP. Praktikum po patofiziologii: Uchebnoe. Ed by prof. A.G. Vasil’eva, N.V. Khaytseva. Saint Petersburg; 2014. (In Russ.)]
- Габбасов З.А., Попов Е.Г., Гаврилов И.Ю., и др. Новый высокочувствительный метод анализа агрегации тромбоцитов // Лабораторное дело. — 1989. — № 10. — С. 15–18. [Gabbasov ZA, Popov EG, Gavrilov IYu, et al. Novyy vysokochuvstvitel’nyy metod analiza agregatsii trombotsitov. Laboratornoe delo. 1989;(10):15-18. (In Russ.)]
- Гаврилова С.А., и др. Нейропротекторный эффект пролинсодержащего дипептида ноопепта на модели мозгового инсульта, вызванного дистальной перевязкой средней мозговой артерии // Экспериментальная и клиническая фармакология. — 2006. — Т. 69. — № 4. — С. 16–18. [Gavrilova SA, et al. Neuroprotective activity of the proline-containing dipeptide noopept on the model of brain ischemia induced by the middle cerebral artery occlusion. Experimental and Clinical Pharmacology. 2006;69(4):16-18. (In Russ.)]
- Степанова, Ю.И., Гончар И.А. Эндотелинзависимые эффекты при цереброваскулярной патологии ишемического генеза // Медицинские новости. — 2013. — № 10. — С. 12–18. [Stepanova YuI, Gonchar IA. Endothelin-dependent effects in cerebrovascular pathology of ischemic genesis. Meditsinskie novosti. 2013;(10):12-18. (In Russ.)]
- Шмонин А.А., Байса А.Е., Мельникова Е.В. Защитные эффекты раннего ишемического прекондиционирования при фокальной ишемии мозга у крыс: роль коллатерального кровообращения // Рос. физиолог. журн. — 2011. — Т. 97. — № 2. — С. 203–214. [Shmonin AA, Baysa AE, Mel’nikova EV. Early ischemic preconditioning against focal transient and permanent brein ischemia in rats: role of collateral circulation. Neuroscience and Behavioral Physiology — Sechenov Physiology Journal. 2011;97(2):203-214. (In Russ.)]
- Born GVR. Aggregation of blood platelets by adenosine diphosfhate and its reversal. Nature. 1962;194:927-9. doi: 10.1038/194927b0.
- Hill NC, Millikan CH, Wakim KG, Sayre GP. Studies in cerebrovascular disease, VII: experimental production of cerebral infarction by intracarotid injection of homologous blood clot: preliminary report. Mayo Clin Proc. 1955;30:625-633.
- Michael T, Gewaltig M, Kojda G. Vasoprotection by nitric oxide: mechanisms and therapeutic potential. Cardiovascular Res. 2002;55:250-260. doi: 10.1016/S0008-6363(02)00327-9.
- Mergenthaler P, Meisel A. Do stroke models model stroke? Disease Models & Mechanisms. 2012;5:718-25.
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