电邮这篇文章 基因多态性在结直肠癌发展中的重要性
- 作者: Kulikov E.1, Sudakov A.1, Nikiforov A.1, Mertsalov S.1, Grigorenko V.1
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隶属关系:
- Ryazan State Medical University
- 期: 卷 28, 编号 2 (2020)
- 页面: 127-134
- 栏目: Original study
- URL: https://journals.rcsi.science/pavlovj/article/view/34899
- DOI: https://doi.org/10.23888/PAVLOVJ2020282127-134
- ID: 34899
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详细
目的：测定MTHFR (Ala222Val), XPD (Lis751Gln), XRCC1 (Arg194Trp), XRCC1 (Arg399Gln), XRCC1 (Arg208His), APE1 (Asp148Glu), hOGG1 (ser326Ces), P53 (Pro47Ser), VEGF (C654G), EGFR(A2073T), TNF(G308A), CHEK2 (Ile157Thr), MMP1 (1607 1G>2G), TIMP1(C53CT) 基因多态性在结直肠癌发生发展中的意思。
材料与方法。我们分析了106例在Regional Clinical Oncology Center 梁赞地区的国家预算机构接受治疗的结肠直肠癌患者。所有患者采用静脉血白细胞DNA提取、聚合酶链反应（PCR）进行基因分型，电泳检测结果。
结果：确诊时患者的年龄与任何被研究基因的多态性均无相关性（p> 0.05）。TNF基因多态性（G308A）与肿瘤分期有统计学意义：其主要纯合基因型G/G在III-IV期患者中更为常见（p=0.047）。G / G TNF等位基因（G308A）与MMP1基因纯合突变等位基因（1607 1G/2G）的存在，与III-IV期诊断患者比例的增加有直接关系。两种多态性的结合在研究组中具有统计学差异（p=0.025）。在10例IV期患者中，有8例发现VEGF基因（C654G）中存在G / G多态性。这种突变的纯合子变异在I期（37.5%）、II期（40%）和III期（37.5%）患者中更少见（p = 0.0147）。
结论。所研究的基因并不影响结肠直肠癌的年龄相关标准，而且在男女患者中发现的频率是一样的，无论年龄组。肿瘤的定位和分化程度也与所研究基因的多态性无关。TNF基因（G308A）的G / A多态性应被认为是有助于降低肿瘤侵袭性的有利标准（p <0.05）。G/G主要基因型的鉴定，特别是结合MMP1基因纯合突变等位基因（1607 1G/2G）是不利因素（p <0.05）。G/G VEGF纯合突变基因型（C654G）的存在与肿瘤快速进展和转移活性直接相关（p <0.05）。
作者简介
Evgeniy Kulikov
Ryazan State Medical University
Email: moroka1695@yandex.ru
ORCID iD: 0000-0003-4926-6646
Researcher ID: S-1851-2016
MD, PhD, Professor, Head of the Department of Oncology
俄罗斯联邦, RyazanAleksey Sudakov
Ryazan State Medical University
Email: moroka1695@yandex.ru
ORCID iD: 0000-0002-6791-9797
SPIN 代码: 9307-0078
Assistant of the Department of Oncology
俄罗斯联邦, RyazanAleksandr Nikiforov
Ryazan State Medical University
Email: moroka1695@yandex.ru
ORCID iD: 0000-0002-7364-7687
SPIN 代码: 8366-5282
MD, PhD, Associate Professor of the Department of Pharmacology with the Course of Pharmacy of the Faculty of Additional Postgraduate Education
俄罗斯联邦, RyazanSergey Mertsalov
Ryazan State Medical University
Email: moroka1695@yandex.ru
ORCID iD: 0000-0002-8804-3034
MD, PhD, Assistant of the Department of Oncology
Vladimir Grigorenko
Ryazan State Medical University
编辑信件的主要联系方式.
Email: moroka1695@yandex.ru
ORCID iD: 0000-0002-4664-5723
SPIN 代码: 7174-5611
Researcher ID: F-8605-2019
Clinical Resident of the Department of Oncology
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