Comparison of cytotoxicity of vascular prostheses in vitro

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Abstract

Aim. To study and compare cytotoxicity of the main types of synthetic prostheses used in arterial reconstructive surgery, including polytetrafluoroethylene (PTFE) and polyethylene-terephthalate (Dacron).

Materials and Methods. On the culture of human umbilical vein endothelial cells (HUVEC) of the 3rd passage, MTS test was conducted that is used in laboratory examinations with attraction of cellular technologies to study cytotoxicity of medical drugs and medical products. The test
implies use of MTS reagent that is 3-(4,5-dimethylthiazol-2-il)-5-(3-carboxymethoxyphenyl)-2-
(4-sulfophenyl)-2H-tetrazolium; additionally phenazine methosulfate (PMS) was used that plays the role of electron-binding reagent. In the experiment, cells were incubated with PTFE and Dacron within 24 hours at 37ᵒC with 5% CO2. For control, HUVEC cultured in the standard growth
medium, were used. In the presence of PMS, MTS was reduced by mitochondrial dehydrogenases of endothelial cells to formazan staining blue. Supernatant of cell cultures was evaluated by
photocolorimetric method on Stat Fax 3200 analyzer (microplate reader) of Awareness technology Inc. Palm City Fl. (USA).

Results. The lowest mean values were noted in Dacron group – 0.21 (0.20-0.22) optical density units, the highest values were noted in the control group – 0.36 (0.35-0.38); parameters in PTFE group were 0.35 (0.33-0.36). In comparison of the groups statistically significant differences were found between the control group and Dacron group (р<0.001), control and PTFE group (р=0.037), Dacron and PTFE (р<0.001). Incubation with Dacron led to suppression of metabolic activity of cells by 41.7% as compared to the control group (р<0.001). Metabolic activity of cells exposed to PTFE, approached that of the control group, that is, it corresponded to the optimal conditions of culturing of endothelial cells in vitro.

Conclusion. In comparison with polyethylene-terephthalate (Dacron), polytetrafluoro-ethylene (PTFE) showed the least suppression of metabolic activity of endothelial cells in vitro.

About the authors

Roman E. Kalinin

Ryazan State Medical University

Email: nina_mzhavanadze@mail.ru
ORCID iD: 0000-0002-0817-9573
SPIN-code: 5009-2318
ResearcherId: M-1554-2016

MD, professor, head. Department of Cardiovascular, Endovascular, Surgical Surgery and Topographic Anatomy

Russian Federation, Ryazan

Igor A. Suchkov

Ryazan State Medical University

Email: nina_mzhavanadze@mail.ru
ORCID iD: 0000-0002-1292-5452
SPIN-code: 6473-8662
Scopus Author ID: M-1180-2016

MD, PhD, Professor, Professor of the Department of Cardiovascular, Endovascular, Operative Surgery and Topographic Anatomy

Russian Federation, Ryazan

Nina D. Mzhavanadze

Ryazan State Medical University

Author for correspondence.
Email: nina_mzhavanadze@mail.ru
ORCID iD: 0000-0001-5437-1112
SPIN-code: 7757-8854
ResearcherId: M-1732-2016

MD, PhD, Associate Professor of the Department of Cardiovascular, Endovascular, Operative Surgery and Topographic Anatomy; Senior Researcher at the Central Research Laboratory

Russian Federation, Ryazan

Natalya V. Korotkova

Ryazan State Medical University

Email: nina_mzhavanadze@mail.ru
ORCID iD: 0000-0001-7974-2450
SPIN-code: 3651-3813
ResearcherId: I-8028-2018

MD, PhD, Associate Professor of the Department of Biochemical Chemistry with Clinical Laboratory Diagnostics Course of the Faculty of Additional Professional Education; Senior Researcher at the Central Research Laboratory

Russian Federation, Ryazan

Aleksandr A. Nikiforov

Ryazan State Medical University

Email: nina_mzhavanadze@mail.ru
ORCID iD: 0000-0002-7364-7687
SPIN-code: 8713-0596

MD, PhD, Associate Professor, Head of the Central Research Laboratory

 
Russian Federation, Ryazan

Ivan Yu. Surov

Ryazan State Medical University

Email: nina_mzhavanadze@mail.ru
ORCID iD: 0000-0002-0794-4544
SPIN-code: 1489-7481

Student of the General Medicine Faculty

Russian Federation, Ryazan

Polina Yu. Ivanova

Ryazan State Medical University

Email: nina_mzhavanadze@mail.ru
ORCID iD: 0000-0001-6943-0277

Student of the General Medicine Faculty

Russian Federation, Ryazan

Anastasiya D. Bozhenova

Ryazan State Medical University

Email: nina_mzhavanadze@mail.ru
ORCID iD: 0000-0002-2790-0303

Student of the General Medicine Faculty

Russian Federation, Ryazan

Ekaterina A. Strelnikova

Ryazan State Medical University

Email: nina_mzhavanadze@mail.ru
ORCID iD: 0000-0002-3370-1095

Student of the Preventive Health Faculty

Russian Federation, Ryazan

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Supplementary files

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2. Fig. 1. The scheme of reduction of MTS to formazan under action of dehydrogenases

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3. Fig. 2. 96-well plate with cell supernatant after incubation with MTS/PMS before measurement of optical density

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4. Fig. 3. Comparison of the optical density values in the studied groups in the experiment on cytotoxicity

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Copyright (c) 2020 Kalinin R.E., Suchkov I.A., Mzhavanadze N.D., Korotkova N.V., Nikiforov A.A., Surov I.Y., Ivanova P.Y., Bozhenova A.D., Strelnikova E.A.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
 


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