Changes in Metabolic Parameters of Rats in Different Age Periods After Prenatal Stress
- Authors: Martyusheva A.S.1, Subbotina A.Y.1, Abramova A.Y.1, Alekseeva I.V.1, Pertsov S.S.1
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Affiliations:
- P. K. Anokhin Research Institute of Normal Physiology
- Issue: Vol 30, No 4 (2022)
- Pages: 471-480
- Section: Original study
- URL: https://journals.rcsi.science/pavlovj/article/view/252585
- DOI: https://doi.org/10.17816/PAVLOVJ106532
- ID: 252585
Cite item
Abstract
INTRODUCTION: Currently, biomedical specialists give special attention to studying the influence of stressogenic factors on an organism of a pregnant woman, the embryonic development of the fetus and the subsequent development of offspring in the early and late ontogenesis. It has been found in a number of studies that prenatal stress can lead to reduction of physical activity and hormonal disorders. The factors leading to disorders in regulation of immune and metabolic homeostasis have been identified. Despite considerable interest in the study of the consequences of prenatal stress, its impact on changes in metabolic intensity in different periods of postnatal ontogenesis in animals of different genders has not been sufficiently studied.
AIM: To study parameters of metabolism intensity in different age periods of postnatal ontogenesis in rats’ offspring of both genders after intrauterine stress load.
MATERIALS AND METHODS: Stress load was modeled in daily forced swimming of pregnant rats at 10–12°C for 5 min. Oxygen consumption, carbon dioxide excretion (ml/h/kg), and heat exchange (kcal/h/kg) in male and female rats were determined using a Phenomaster automated modular unit (TSE Systems GmbH, Germany) on the 21st, 30th and 60th day of the ontogenesis. Statistical significance of differences between the studied parameters was assessed using Mann-Whitney U-test for independent groups.
RESULTS: The study parameters of intact animals practically did not differ in the suckling (the 21st day) and infantile period (the 30th day), but significantly decreased in the juvenile period of development (the 60th day). It was found that the intrauterine stress did not lead to statistically significant changes in the absolute values of metabolic parameters as compared to the control, but affected the period of appearance of age-related variations of these parameters in male rats. In these animals, the volumes of oxygen consumption, carbon dioxide excretion, and the level of heat radiation reduced as early as in the infantile period (as compared to 21-day-old rats) and reached the minimum level by the 60th day of the postnatal ontogenesis. In female rats subjected to intrauterine stress, changes in the study parameters in different periods of life were similar to those of intact animals.
CONCLUSION: Therefore, prenatal stress modeled by swimming of female parents in cold water, has a modulating effect on age-related dynamics of metabolic parameters of male rats, which was manifested by the decrease in these parameters in the earlier period of development.
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##article.viewOnOriginalSite##About the authors
Anna S. Martyusheva
P. K. Anokhin Research Institute of Normal Physiology
Author for correspondence.
Email: org.otd.niinf@yandex.ru
ORCID iD: 0000-0001-8847-0178
SPIN-code: 8989-7409
Russian Federation, Moscow
Anastasiya Yu. Subbotina
P. K. Anokhin Research Institute of Normal Physiology
Email: rat-stress@mail.ru
ORCID iD: 0000-0002-2665-9917
SPIN-code: 8572-1590
Russian Federation, Moscow
Anastasiya Yu. Abramova
P. K. Anokhin Research Institute of Normal Physiology
Email: nansy71@mail.ru
ORCID iD: 0000-0001-5940-3056
SPIN-code: 1790-3941
MD, Dr. Sci. (Med.)
Russian Federation, MoscowIrina V. Alekseeva
P. K. Anokhin Research Institute of Normal Physiology
Email: kiv24irina@mail.ru
ORCID iD: 0000-0001-9236-5143
SPIN-code: 3539-5592
Cand. Sci. (Biol.)
Russian Federation, MoscowSergey S. Pertsov
P. K. Anokhin Research Institute of Normal Physiology
Email: s.pertsov@mail.ru
ORCID iD: 0000-0001-5530-4990
SPIN-code: 3876-0513
MD, Dr. Sci. (Med.), Professor
Russian Federation, MoscowReferences
- Belyayeva LE, Fedchenko AN, Ligetskaya IV, et al. Vliyaniye kombinirovannogo prenatal’nogo stressa na fizicheskuyu vynoslivost’, bolevuyu chuvstvitel’nost’ i povedeniye krys. Vestnik Vitebskogo Gosudarstvennogo Meditsinskogo Universiteta. 2015;14(1):26–33. (In Russ).
- Nikolaeva IV, Belolyubskaya DS. Prenatal infl uence of emotional stress on the development of brain, adrenal and gonads of rats in postnatal ontogeny. Vestnik of the North-Esatern Federal University. 2012;9(1):68–73. (In Russ).
- Tamashiro KLK, Terrillion CE, Hyun J, et al. Prenatal stress or high-fat diet increases susceptibility to diet-induced obesity in rat offspring. Diabetes. 2009;58(5):1116–25. doi: 10.2337/db08-1129
- Lian S, Li W, Wang D, et al. Effects of prenatal cold stress on maternal serum metabolomics in rats. Life Sciences. 2020;246:117432. doi: 10.1016/j.lfs.2020.117432
- Pertsov SS, Alekseeva IV, Abramova AYu, et al. Metabolic parameters in rats at various stages of the post-stress period under conditions of antigenic exposure by administration of lipopoly-saccharide. Russian Journal of Physiology. 2021;107(3):321–31. (In Russ). doi: 10.31857/S0869813921030080
- Teryayeva NB. Stress: metabolicheskiye osnovy adaptatsii i patologiya serdechno-sosudistoy sistemy. Kreative Cardiology. 2008;(1): 24–30. (In Russ).
- Pertsov SS, Gurcovskii BV, Abramova AYu, et al. Dynamics of metabolic parameters in rats during repeated exposure to modulated low-intensity uhf radiation. Bulletin of Experimental Biology and Medicine. 2018;165(4): 419–23. (In Russ). doi: 10.1007/s10517-018-4184-9
- Volodina MA, Sebentsova EA, Levitskaia NG, et al. Study of long-lasting effects of acute prenatal stress induced forced swimming. Zhurnal Vysshey Nervnoi Deiatelnosti imeni I P Pavlova. 2010;60(6):730–9. (In Russ).
- Batuyev AS. U istokov prenatal’noy psikhologii. Development of Personality. 2008;(1):113–23. (In Russ).
- Zapadnyuk IP, Zapadnyuk VI, Zakhariya EA, et al. Laboratornyye zhivotnyye: razvedeniye, soderzhaniye, ispol’zovaniye v eksperimente. 3rd ed. Kiyev: Vishcha shkola. Golovnoye izdatelstvo; 1983. (In Russ).
- Barker DJP, Lampl M. Commentary: The meaning of thrift. International Journal of Epidemiology. 2013;42(5):1229–30. doi: 10.1093/ije/dyt131
- Dil’man VM. Bol’shiye biologicheskiye chasy. Vvedeniye v integral’nuyu meditsinu. Moscow: Znaniye; 1982. (In Russ).
- Nikitina YuV, Mukhina IV. Age-related changes of oxidizing processes in cerebral tissue and blood of rats. Vestnik Nizhegorodskogo Gosudarstvennogo Universiteta imeni N.I. Lobachevskogo. 2009;(6):102–6. (In Russ).
- Dil’man VM, Tsyrlina EV. Vliyaniye vnutrenney sredy na razvitiye organizma na vozniknoveniye i razvitiye opukholey. In: Obshchaya onkologiya. Moscow: Meditsina; 1989. P. 119–42. (In Russ).
- Molendijk ML, de Kloet ER. Immobility in the forced swim test is adaptive and does not reflect depression. Psychoneuroendocrinology. 2015;62:389–91. doi: 10.1016/j.psyneuen.2015.08.028
- Sashkov VA, Sel’verova NB, Ermakova IV. Vozrastnyye i polovyye osobennosti povedeniya i urovnya steroidnykh gormonov v mozge u krys v neonatal’nom i rannem postnatal’nom periode razvitiya. Novyye Issledovaniya. 2008;(1):52–61. (In Russ).
- Ryzhavskii BYa, Sokolova TV. Effect of emotional stress of pregnant rats on brain development indices of their 21-days old posterity. Dal'nevostochnyy Meditsinskiy Zhurnal. 2002;(4):28–31. (In Russ).
- Ordyan NE, Pivina SG, Baranova KA, et al. Sex-Dependent Modulation of the Hypothalamic-Pituitary-Adrenal Axis Stress Reactivity After Prenatal Stress in Rats: Relevance of the Brain Corticosteroid Receptors Expression. Russian Journal of Physiology. 2020;106(6):740–55. (In Russ). doi: 10.31857/S0869813920060096
- Murmu MS, Salomon S, Biala Y, et al. Changes of spine density and dendritic complexity in the prefrontal cortex in offspring of mothers exposed to stress during pregnancy. The European Journal of Neuroscience. 2006;24(5):1477–87. doi: 10.1111/j.1460-9568.2006.05024.x